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SIGLEC10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SIGLEC10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SIGLEC10 antibodies →
$504.00each
Catalog Number: LS-G130066-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIGLEC10 (NM_001171157), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SIGLEC-10 | Siglec-like gene 2 | Siglec-like protein 2 | SIGLEC10 | PRO940 | SLG2
Origin Species
Human
SwissProt
Q96LC7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLPLLLSSL LGGSQAMDGR FWIRVQESVM VPEGLCISVP CSFSYPRQDW TGSTPAYGYW
61FKAVTETTKG APVATNHQSR EVEMSTRGRF QLTGDPAKGN CSLVIRDAQM QDESQYFFRV
121ERGSYVRYNF MNDGFFLKVT ALTQKPDVYI PETLEPGQPV TVICVFNWAF EECPPPSFSW
181TGAALSSQGT KPTTSHFSVL SFTPRPQDHN TDLTCHVDFS RKGVSAQRTV RLRVAYAPRD
241LVISISRDNT PALEPQPQGN VPYLEAQKGQ FLRLLCAADS QPPATLSWVL QNRVLSSSHP
301WGPRPLGLEL PGVKAGDSGR YTCRAENRLG SQQRALDLSV QYPPENLRVM VSQANRTVLE
361NLGNGTSLPV LEGQSLCLVC VTHSSPPARL SWTQRGQVLS PSQPSDPGVL ELPRVQVEHE
421GEFTCHARHP LGSQHVSLSL SVHYKKGLIS TAFSNGAFLG IGITALLFLC LALIIMKILP
481KRRTQTETPR PRFSRHSTIL DYINVVPTAG PLAQKRNQKA TPNSPRTPLP PGAPSPESKK
541NQKKQYQLPS FPEPKSSTQA PESQESQEEL HYATLNFPGV RPRPEARMPK GTQADYAEVK
601FQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

602 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.