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SIGLEC10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SIGLEC10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SIGLEC10 antibodies →
$504.00each
Catalog Number: LS-G130116-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIGLEC10 (NM_001171156), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SIGLEC-10 | Siglec-like gene 2 | Siglec-like protein 2 | SIGLEC10 | PRO940 | SLG2
Origin Species
Human
SwissProt
Q96LC7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLPLLLSSL LGGSQAMDGR FWIRVQESVM VPEGLCISVP CSFSYPRQDW TGSTPAYGYW
61FKAVTETTKG APVATNHQSR EVEMSTRGRF QLTGDPAKGN CSLVIRDAQM QDESQYFFRV
121ERGSYVRYNF MNDGFFLKVT VLSFTPRPQD HNTDLTCHVD FSRKGVSAQR TVRLRVAYAP
181RDLVISISRD NTPALEPQPQ GNVPYLEAQK GQFLRLLCAA DSQPPATLSW VLQNRVLSSS
241HPWGPRPLGL ELPGVKAGDS GRYTCRAENR LGSQQRALDL SVQYPPENLR VMVSQANRTV
301LENLGNGTSL PVLEGQSLCL VCVTHSSPPA RLSWTQRGQV LSPSQPSDPG VLELPRVQVE
361HEGEFTCHAR HPLGSQHVSL SLSVHYSPKL LGPSCSWEAE GLHCSCSSQA SPAPSLRWWL
421GEELLEGNSS QDSFEVTPSS AGPWANSSLS LHGGLSSGLR LRCEAWNVHG AQSGSILQLP
481DKKGLISTAF SNGAFLGIGI TALLFLCLAL IIMKILPKRR TQTETPRPRF SRHSTILDYI
541NVVPTAGPLA QKRNQKATPN SPRTPLPPGA PSPESKKNQK KQYQLPSFPE PKSSTQAPES
601QESQEELHYA TLNFPGVRPR PEARMPKGTQ ADYAEVKFQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

639 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.