LSBio
MITF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MITF Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G123454-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MITF (NM_000248), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BHLHe32 | CMM8 | MI | MITF | Waardenburg syndrome | type 2A | WS2 | WS2A
Origin Species
Human
NCBI GenBank
NM_000248
SwissProt
O75030

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLEMLEYNHY QVQTHLENPT KYHIQQAQRQ QVKQYLSTTL ANKHANQVLS LPCPNQPGDH
61VMPPVPGSSA PNSPMAMLTL NSNCEKEGFY KFEEQNRAES ECPGMNTHSR ASCMQMDDVI
121DDIISLESSY NEEILGLMDP ALQMANTLPV SGNLIDLYGN QGLPPPGLTI SNSCPANLPN
181IKRELTACIF PTESEARALA KERQKKDNHN LIERRRRFNI NDRIKELGTL IPKSNDPDMR
241WNKGTILKAS VDYIRKLQRE QQRAKELENR QKKLEHANRH LLLRIQELEM QARAHGLSLI
301PSTGLCSPDL VNRIIKQEPV LENCSQDLLQ HHADLTCTTT LDLTDGTITF NNNLGTGTEA
361NQAYSVPTKM GSKLEDILMD DTLSPVGVTD PLLSSVSPGA SKTSSRRSSM SMEETEHTC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

419 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.