Western blot analysis of extracts of various cell lines, using MITF antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

MITF Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C331388-20

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Overview

Description: MITF antibody LS-C331388 is an unconjugated rabbit polyclonal antibody to MITF from human. It is reactive with human and mouse. Validated for WB.
Application: IHC, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: BHLHe32 | CMM8 | MI | MITF | Waardenburg syndrome, type 2A | WS2 | WS2A
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: O75030
NCBI GenBank: NM_000248 | NP_000239.1
SwissProt: O75030

Target

Target: Human MITF
Antigen Species: Human
Epitope: Human MITF
Gene Name: MITF

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MITF (NP_937801.1). MLEMLEYNHYQVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPGSSAPNSPMAMLTLNSNCEKEGFYKFEEQNRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQGLPPPGLTISNSCPANLPNIKRELTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKELENRQKKLEHANRHLLLRIQ
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)