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Polyclonal Rabbit anti-Human MITF Antibody (phospho-Ser180/Ser73, WB)

Western blot analysis of lysates from COS7 cells, using MITF (Phospho-Ser180/73) Antibody. The lane on the right is blocked with the phospho peptide.

Polyclonal Rabbit anti-Human MITF Antibody (phospho-Ser180/Ser73, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse
Format:Liquid
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$341.00each
Catalog Number: LS-C199259-50

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Overview

Description
MITF antibody LS-C199259 is an unconjugated rabbit polyclonal antibody to MITF (pSer180/Ser73) from human. It is reactive with human and mouse. Validated for Peptide-ELISA and WB. Cited in 2 publications.
Application
ELISA, IHC, WB
Reactivity
Hu, Ms
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BHLHe32 | CMM8 | MI | MITF | Waardenburg syndrome, type 2A | WS2 | WS2A
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
O75030
SwissProt
O75030

Target

Target
Human MITF
Antigen Species
Hu
Epitope
MITF (Phospho-Ser180/73) Antibody detects endogenous levels of MITF only when phosphorylated at serine180/73.
Gene Name
MITF

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Synthetic peptide derived from human MITF around the phosphorylation site of Ser180/73. (AA Range: 151-200)
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS|150 mM NaCl
Mitochondrial dynamics regulate melanogenesis through proteasomal degradation of MITF via ROS-ERK activation. Kim ES, Park SJ, Goh MJ, Na YJ, Jo DS, Jo YK, Shin JH, Choi ES, Lee HK, Kim JY, Jeon HB, Kim JC, Cho DH. Pigment cell & melanoma research. Nov;27:1051-62. PubMed: 25065405

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