LSBio
Human TNF Alpha Protein (Recombinant Human IgG1 Fc, N-terminus)

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.5-3ng/mL.

Human TNF Alpha Protein (Recombinant Human IgG1 Fc, N-terminus)

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Expression SystemHuman Embryonic Kidney 293
Format:Lyophilized
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$450.00each
Catalog Number: LS-G47237-100

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Overview

Description
TNF Alpha Protein LS-G47237 is a Recombinant Human TNF Alpha produced in HEK 293 Cells Val77-Leu233 with Human IgG1 Fc, N-terminus tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | macrophage-derived | monocyte-derived | TNFSF2
Origin Species
Hu
NCBI GenBank
NM_000594
SwissProt
P01375

Protein

Expression System
HEK293
Fusion Tag
hFc
Molecular Weight
The recombinant human TNF consists 417 amino acids and predicts a molecular mass of 45.8 kDa.
Protein Type
Recombinant
Amino Acid Sequence
1Val77-Leu2 33

12 aa

Format

Purity
>90%
Conjugate/Label
Unconj
Endotoxin Level
< 1.0 EU/µg
Format
Lyophilized
Formulation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Purity Measurement Method
SDS-PAGE

Storage & Handling

Storage Conditions
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.