LSBio
TNF Alpha Antibody

TNF Alpha Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Neutralization, Radioimmunoassay, Western Blot
Reactivity:Mouse
Format:Liquid
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$579.00each
Catalog Number: LS-C744655-100

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Overview

Description
TNF Alpha antibody LS-C744655 is an unconjugated rabbit polyclonal antibody to mouse TNF Alpha. Validated for ELISA, IHC, IP, Neut, RIA and WB.
Application
ELISA, IHC, IP, Neut, RIA, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Recommended Dilution: ELISA
1:1000 - 1:5000
Recommended Dilution: IHC
1:100 - 1:500
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P01375
NCBI GenBank
NM_000594 | NP_000585.2
SwissProt
P01375

Target

Target
Mouse TNF Alpha
Antigen Species
Mouse
Epitope
The antiserum is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Gene Name
TNF

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum
Concentration
85 mg/mL
Volume
100 Microliter
Preservative
No
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.