LSBio
TNF Alpha Antibody

Western blot of Anti-Human TNF-a (RABBIT) Antibody. Lane 1: 250ng human recombinant TNF. Lane 2: 500ng human recombinant TNF. Lane 3: 1000ng human recombinant TNF. Membrane blocked in Blotto p/n B501-0500 for 30 min RT. Primary antibody: Rb-a-TNF alpha added at 1:1000 in overnight at 4°C. Secondary Antibody : Gt-a-Rb HRP p/n 611-103-122 added at 1:20,000 in 5% Blotto for 30 min RT. (5 second exposure).

TNF Alpha Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Neutralization, Western Blot
Reactivity:Human
Format:Liquid
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$579.00each
Catalog Number: LS-C745236-100

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Overview

Description
TNF Alpha antibody LS-C745236 is an unconjugated rabbit polyclonal antibody to human TNF Alpha. Validated for ELISA, IF, IHC, Neut and WB.
Application
ELISA, IF, IHC, Neut, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Recommended Dilution: ELISA
1:1000 - 1:5000
Recommended Dilution: IHC
1:100 - 1:500
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P01375
NCBI GenBank
NM_000594 | NP_000585.2
SwissProt
P01375

Target

Target
Human TNF Alpha
Antigen Species
Human
Epitope
This antibody is primarily directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFβ (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Gene Name
TNF

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFa.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
No
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.