Insulin-mimetic effects on stimulated differentiating 3T3-L1 cells. 10 ug/ml iNampt (human) (rec.) (His) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1 uM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.

Human NAMPT / Visfatin Protein (Recombinant His)

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Expression System	Escherichia coli (Prokaryotic)
Format:	Lyophilized

$466.00each

Catalog Number: LS-G3729-50

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Overview

Description: Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.
Category: Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names: PBEF | Pre-B cell-enhancing factor | VISFATIN | VF | NAmPRTase | NAMPT | PBEF1
Origin Species: Human
NCBI GenBank: NM_005746|NP_005737.1
SwissProt: P43490

Protein

Expression System: Escherichia coli (Prokaryotic)
Fusion Tag: Hexahistidine Tag
Molecular Weight: ~52kDa (SDS-PAGE)
Protein Type: Recombinant
Amino Acid Sequence

Format

Purity: >90%
Conjugate/Label: Unconjugated
Endotoxin Level: < 1.0 EU/µg
Format: Lyophilized
Formulation: Lyophilized, Salt free.
Purity Measurement Method: SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions: Store lyophilized at -20°C for up to 1 year. Once reconstituted store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.