LSBio
Mouse NAMPT / Visfatin Protein (Recombinant His)

Insulin-mimetic effects on stimulated differentiating 3T3-L1 cells. 10 ug/ml Nampt (mouse) (rec.) (His) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1 uM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.

Mouse NAMPT / Visfatin Protein (Recombinant His)

Expression SystemEscherichia coli (Prokaryotic)
Format:Lyophilized
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$466.00each
Catalog Number: LS-G3731-50

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Overview

Description
Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
PBEF | Pre-B cell-enhancing factor | VISFATIN | VF | NAmPRTase | NAMPT | PBEF1
Origin Species
Mouse
SwissProt
P43490

Protein

Expression System
Escherichia coli (Prokaryotic)
Fusion Tag
Hexahistidine Tag
Molecular Weight
~52kDa (SDS-PAGE)
Protein Type
Recombinant
Amino Acid Sequence
1aa 1-491

8 aa

Format

Purity
>90%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Lyophilized
Formulation
Lyophilized from PBS, pH 7.2
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.