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UBE2O Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UBE2O Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UBE2O antibodies →
$725.00each
Catalog Number: LS-G110501-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human E2 230K(UBE2O) (NM_022066), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1734 | Ubiquitin-protein ligase O | Ubiquitin carrier protein O | E2-230K | UBE2O
Origin Species
Human
NCBI GenBank
NM_022066
SwissProt
Q9C0C9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
141.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADPAAPTPA APAPAQAPAP APEAVPAPAA APVPAPAPAS DSASGPSSDS GPEAGSQRLL
61FSHDLVSGRY RGSVHFGLVR LIHGEDSDSE GEEEGRGSSG CSEAGGAGHE EGRASPLRRG
121YVRVQWYPEG VKQHVKETKL KLEDRSVVPR DVVRHMRSTD SQCGTVIDVN IDCAVKLIGT
181NCIIYPVNSK DLQHIWPFMY GDYIAYDCWL GKVYDLKNQI ILKLSNGARC SMNTEDGAKL
241YDVCPHVSDS GLFFDDSYGF YPGQVLIGPA KIFSSVQWLS GVKPVLSTKS KFRVVVEEVQ
301VVELKVTWIT KSFCPGGTDS VSPPPSVITQ ENLGRVKRLG CFDHAQRQLG ERCLYVFPAK
361VEPAKIAWEC PEKNCAQGEG SMAKKVKRLL KKQVVRIMSC SPDTQCSRDH SMEDPDKKGE
421SKTKSEAESA SPEETPDGSA SPVEMQDEGA EEPHEAGEQL PPFLLKEGRD DRLHSAEQDA
481DDEAADDTDD TSSVTSSASS TTSSQSGSGT SRKKSIPLSI KNLKRKHKRK KNKITRDFKP
541GDRVAVEVVT TMTSADVMWQ DGSVECNIRS NDLFPVHHLD NNEFCPGDFV VDKRVQSCPD
601PAVYGVVQSG DHIGRTCMVK WFKLRPSGDD VELIGEEEDV SVYDIADHPD FRFRTTDIVI
661RIGNTEDGAP HKEDEPSVGQ VARVDVSSKV EVVWADNSKT IILPQHLYNI ESEIEESDYD
721SVEGSTSGAS SDEWEDDSDS WETDNGLVED EHPKIEEPPI PPLEQPVAPE DKGVVISEEA
781ATAAVQGAVA MAAPMAGLME KAGKDGPPKS FRELKEAIKI LESLKNMTVE QLLTGSPTSP
841TVEPEKPTRE KKFLDDIKKL QENLKKTLDN VAIVEEEKME AVPDVERKED KPEGQSPVKA
901EWPSETPVLC QQCGGKPGVT FTSAKGEVFS VLEFAPSNHS FKKIEFQPPE AKKFFSTVRK
961EMALLATSLP EGIMVKTFED RMDLFSALIK GPTRTPYEDG LYLFDIQLPN IYPAVPPHFC
1021YLSQCSGRLN PNLYDNGKVC VSLLGTWIGK GTERWTSKSS LLQVLISIQG LILVNEPYYN
1081EAGFDSDRGL QEGYENSRCY NEMALIRVVQ SMTQLVRRPP EVFEQEIRQH FSTGGWRLVN
1141RIESWLETHA LLEKAQALPN GVPKASSSPE PPAVAELSDS GQQEPEDGGP APGEASQGSD
1201SEGGAQGLAS ASRDHTDQTS ETAPDASVPP SVKPKKRRKS YRSFLPEKSG YPDIGFPLFP
1261LSKGFIKSIR GVLTQFRAAL LEAGMPECTE DK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1292 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.