UBE2O Antibody
Catalog Number: LS-C496747
2 sizes / from $293.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human UBE2O Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87402-20
Catalog Number: LS-G87402
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human E2 230K(UBE2O) (NM_022066) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1734 | Ubiquitin-protein ligase O | Ubiquitin carrier protein O | E2-230K | UBE2O
- Origin Species
- Human
- NCBI GenBank
- NM_022066
- SwissProt
- Q9C0C9
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 141.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADPAAPTPA APAPAQAPAP APEAVPAPAA APVPAPAPAS DSASGPSSDS GPEAGSQRLL61FSHDLVSGRY RGSVHFGLVR LIHGEDSDSE GEEEGRGSSG CSEAGGAGHE EGRASPLRRG121YVRVQWYPEG VKQHVKETKL KLEDRSVVPR DVVRHMRSTD SQCGTVIDVN IDCAVKLIGT181NCIIYPVNSK DLQHIWPFMY GDYIAYDCWL GKVYDLKNQI ILKLSNGARC SMNTEDGAKL241YDVCPHVSDS GLFFDDSYGF YPGQVLIGPA KIFSSVQWLS GVKPVLSTKS KFRVVVEEVQ301VVELKVTWIT KSFCPGGTDS VSPPPSVITQ ENLGRVKRLG CFDHAQRQLG ERCLYVFPAK361VEPAKIAWEC PEKNCAQGEG SMAKKVKRLL KKQVVRIMSC SPDTQCSRDH SMEDPDKKGE421SKTKSEAESA SPEETPDGSA SPVEMQDEGA EEPHEAGEQL PPFLLKEGRD DRLHSAEQDA481DDEAADDTDD TSSVTSSASS TTSSQSGSGT SRKKSIPLSI KNLKRKHKRK KNKITRDFKP541GDRVAVEVVT TMTSADVMWQ DGSVECNIRS NDLFPVHHLD NNEFCPGDFV VDKRVQSCPD601PAVYGVVQSG DHIGRTCMVK WFKLRPSGDD VELIGEEEDV SVYDIADHPD FRFRTTDIVI661RIGNTEDGAP HKEDEPSVGQ VARVDVSSKV EVVWADNSKT IILPQHLYNI ESEIEESDYD721SVEGSTSGAS SDEWEDDSDS WETDNGLVED EHPKIEEPPI PPLEQPVAPE DKGVVISEEA781ATAAVQGAVA MAAPMAGLME KAGKDGPPKS FRELKEAIKI LESLKNMTVE QLLTGSPTSP841TVEPEKPTRE KKFLDDIKKL QENLKKTLDN VAIVEEEKME AVPDVERKED KPEGQSPVKA901EWPSETPVLC QQCGGKPGVT FTSAKGEVFS VLEFAPSNHS FKKIEFQPPE AKKFFSTVRK961EMALLATSLP EGIMVKTFED RMDLFSALIK GPTRTPYEDG LYLFDIQLPN IYPAVPPHFC1021YLSQCSGRLN PNLYDNGKVC VSLLGTWIGK GTERWTSKSS LLQVLISIQG LILVNEPYYN1081EAGFDSDRGL QEGYENSRCY NEMALIRVVQ SMTQLVRRPP EVFEQEIRQH FSTGGWRLVN1141RIESWLETHA LLEKAQALPN GVPKASSSPE PPAVAELSDS GQQEPEDGGP APGEASQGSD1201SEGGAQGLAS ASRDHTDQTS ETAPDASVPP SVKPKKRRKS YRSFLPEKSG YPDIGFPLFP1261LSKGFIKSIR GVLTQFRAAL LEAGMPECTE DKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1292 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
