Western blot analysis of extracts of various cell lines, using UBE2O antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

UBE2O Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$293.00each

Catalog Number: LS-C496747-20

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Overview

Description: UBE2O antibody LS-C496747 is an unconjugated rabbit polyclonal antibody to UBE2O from human. It is reactive with human and mouse. Validated for WB.
Application: WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: KIAA1734 | Ubiquitin-protein ligase O | Ubiquitin carrier protein O | E2-230K | UBE2O
Recommended Dilution: WB: 1:1000 - 1:4000
UniProt Number: Q9C0C9
NCBI GenBank: NM_022066 | NP_071349.3
SwissProt: Q9C0C9

Target

Target: Human UBE2O
Antigen Species: Human
Gene Name: UBE2O

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3). LVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)