Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPTA1 / Alpha Spectrin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117536-100
Catalog Number: LS-G117536
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human alpha 1 Spectrin(SPTA1) (NM_003126), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EL2 | Erythroid alpha-spectrin | HS3 | Alpha-I spectrin | SPTA1 | HPP | SPH3 | SPTA
- Origin Species
- Human
- NCBI GenBank
- NM_003126
- SwissProt
- P02549
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 281.06 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEQFPKETVV ESSGPKVLET AEEIQERRQE VLTRYQSFKE RVAERGQKLE DSYHLQVFKR61DADDLGKWIM EKVNILTDKS YEDPTNIQGK YQKHQSLEAE VQTKSRLMSE LEKTREERFT121MGHSAHEETK AHIEELRHLW DLLLELTLEK GDQLLRALKF QQYVQECADI LEWIGDKEAI181ATSVELGEDW ERTEVLHKKF EDFQVELVAK EGRVVEVNQY ANECAEENHP DLPLIQSKQN241EVNAAWERLR GLALQRQKAL SNAANLQRFK RDVTEAIQWI KEKEPVLTSE DYGKDLVASE301GLFHSHKGLE RNLAVMSDKV KELCAKAEKL TLSHPSDAPQ IQEMKEDLVS SWEHIRALAT361SRYEKLQATY WYHRFSSDFD ELSGWMNEKT AAINADELPT DVAGGEVLLD RHQQHKHEID421SYDDRFQSAD ETGQDLVNAN HEASDEVREK MEILDNNWTA LLELWDERHR QYEQCLDFHL481FYRDSEQVDS WMSRQEAFLE NEDLGNSLGS AEALLQKHED FEEAFTAQEE KIITVDKTAT541KLIGDDHYDS ENIKAIRDGL LARRDALREK AATRRRLLKE SLLLQKLYED SDDLKNWINK601KKKLADDEDY KDIQNLKSRV QKQQVFEKEL AVNKTQLENI QKTGQEMIEG GHYASDNVTT661RLSEVASLWE ELLEATKQKG TQLHEANQQL QFENNAEDLQ RWLEDVEWQV TSEDYGKGLA721EVQNRLRKHG LLESAVAARQ DQVDILTDLA AYFEEIGHPD SKDIRARQES LVCRFEALKE781PLATRKKKLL DLLHLQLICR DTEDEEAWIQ ETEPSATSTY LGKDLIASKK LLNRHRVILE841NIASHEPRIQ EITERGNKMV EEGHFAAEDV ASRVKSLNQN MESLRARAAR RQNDLEANVQ901FQQYLADLHE AETWIREKEP IVDNTNYGAD EEAAGALLKK HEAFLLDLNS FGDSMKALRN961QANACQQQQA APVEGVAGEQ RVMALYDFQA RSPREVTMKK GDVLTLLSSI NKDWWKVEAA1021DHQGIVPAVY VRRLAHDEFP MLPQRRREEP GNITQRQEQI ENQYRSLLDR AEERRRRLLQ1081RYNEFLLAYE AGDMLEWIQE KKAENTGVEL DDVWELQKKF DEFQKDLNTN EPRLRDINKV1141ADDLLFEGLL TPEGAQIRQE LNARWGSLQR LADEQRQLLG SAHAVEVFHR EADDTKEQIE1201KKCQALSAAD PGSDLFSVQA LQRRHEGFER DLVPLGDKVT ILGETAERLS ESHPDATEDL1261QRQKMELNEA WEDLQGRTKD RKESLNEAQK FYLFLSKARD LQNWISSIGG MVSSQELAED1321LTGIEILLER HQEHRADMEA EAPTFQALED FSAELIDSGH HASPEIEKKL QAVKLERDDL1381EKAWEKRKKI LDQCLELQMF QGNCDQVESR MVARENSLRS DDKSSLDSLE ALMKKRDDLD1441KAITAQEGKI TDLEHFAESL IADEHYAKEE IATRLQRVLD RWKALKAQLI DERTKLGDYA1501NLKQFYRDLE ELEEWISEML PTACDESYKD ATNIQRKYLK HQTFAHEVDG RSEQVHGVIN1561LGNSLIERSC DGNEEAMKEQ LEQLKEHWDH LLERTNDKGK KLNEASRQQR FNTSIRDFEF1621WLSEAETLLA MKDQARDLAS AGNLLKKHQL LEREMLARED ALKDLNTLAE DLLSSGTFNV1681DQIVKKKDNV NKRFLNVQEL AAAHHEKLKE AYALFQFFQD LDDEESWIEE KLIRVSSQDY1741GRDLQGVQNL LKKHKRLEGE LVAHEPAIQN VLDMAEKLKD KAAVGQEEIQ LRLAQFVEHW1801EKLKELAKAR GLKLEESLEY LQFMQNAEEE EAWINEKNAL AVRGDCGDTL AATQSLLMKH1861EALENDFAVH ETRVQNVCAQ GEDILNKVLH EESQNKEISS KIEALNEKTP SLAKAIAAWK1921LQLEDDYAFQ EFNWKADVVE AWIADKETSL KTNGNGADLG DFLTLLAKQD TLDASLQSFQ1981QERLPEITDL KDKLISAQHN QSKAIEERYA ALLKRWEQLL EASAVHRQKL LEKQLPLQKA2041EDLFVEFAHK ASALNNWCEK MEENLSEPVH CVSLNEIRQL QKDHEDFLAS LARAQADFKC2101LLELDQQIKA LGVPSSPYTW LTVEVLERTW KHLSDIIEER EQELQKEEAR QVKNFEMCQE2161FEQNASTFLQ WILETRAYFL DGSLLKETGT LESQLEANKR KQKEIQAMKR QLTKIVDLGD2221NLEDALILDI KYSTIGLAQQ WDQLYQLGLR MQHNLEQQIQ AKDIKGVSEE TLKEFSTIYK2281HFDENLTGRL THKEFRSCLR GLNYYLPMVE EDEHEPKFEK FLDAVDPGRK GYVSLEDYTA2341FLIDKESENI KSSDEIENAF QALAEGKSYI TKEDMKQALT PEQVSFCATH MQQYMDPRVE2401AISLAMTTLA SPIPTLATNK QLLVDRRKSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
2429 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.