Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPHK / SPHK1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104047-100
Catalog Number: LS-G104047
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPHK1 (NM_182965), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SK1 | Sphingosine kinase 1 | SPHK | SPHK1 | SK 1 | SPK 1 | Sphingosine kinase type 1 | SPK
- Origin Species
- Human
- NCBI GenBank
- NM_021972
- SwissProt
- Q9NYA1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSAQVLGFLR SWTPLPLAAP RGPAAAGNDA GAPTATAPGG EGEPHSRPCD ARLGSTDKEL61KAGAAATGSA PTAPGTPWQR EPRVEVMDPA GGPRGVLPRP CRVLVLLNPR GGKGKALQLF121RSHVQPLLAE AEISFTLMLT ERRNHARELV RSEELGRWDA LVVMSGDGLM HEVVNGLMER181PDWETAIQKP LCSLPAGSGN ALAASLNHYA GYEQVTNEDL LTNCTLLLCR RLLSPMNLLS241LHTASGLRLF SVLSLAWGFI ADVDLESEKY RRLGEMRFTL GTFLRLAALR TYRGRLAYLP301VGRVGSKTPA SPVVVQQGPV DAHLVPLEEP VPSHWTVVPD EDFVLVLALL HSHLGSEMFA361APMGRCAAGV MHLFYVRAGV SRAMLLRLFL AMEKGRHMEY ECPYLVYVPV VAFRLEPKDG421KGVFAVDGEL MVSEAVQGQV HPNYFWMVSG CVEPPPSWKP QQMPPPEEPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
470 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.