LSBio
Human SPHK / SPHK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SPHK / SPHK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G83869-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SPHK1 (NM_182965) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SK1 | Sphingosine kinase 1 | SPHK | SPHK1 | SK 1 | SPK 1 | Sphingosine kinase type 1 | SPK
Origin Species
Human
NCBI GenBank
NM_021972
SwissProt
Q9NYA1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAQVLGFLR SWTPLPLAAP RGPAAAGNDA GAPTATAPGG EGEPHSRPCD ARLGSTDKEL
61KAGAAATGSA PTAPGTPWQR EPRVEVMDPA GGPRGVLPRP CRVLVLLNPR GGKGKALQLF
121RSHVQPLLAE AEISFTLMLT ERRNHARELV RSEELGRWDA LVVMSGDGLM HEVVNGLMER
181PDWETAIQKP LCSLPAGSGN ALAASLNHYA GYEQVTNEDL LTNCTLLLCR RLLSPMNLLS
241LHTASGLRLF SVLSLAWGFI ADVDLESEKY RRLGEMRFTL GTFLRLAALR TYRGRLAYLP
301VGRVGSKTPA SPVVVQQGPV DAHLVPLEEP VPSHWTVVPD EDFVLVLALL HSHLGSEMFA
361APMGRCAAGV MHLFYVRAGV SRAMLLRLFL AMEKGRHMEY ECPYLVYVPV VAFRLEPKDG
421KGVFAVDGEL MVSEAVQGQV HPNYFWMVSG CVEPPPSWKP QQMPPPEEPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

470 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.