Western blot of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
SPHK / SPHK1 Antibody (aa286-315)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate, Rat |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C100769-200
Catalog Number: LS-C100769
For bulk orders or custom formulations:
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- Description
- SPHK1 antibody LS-C100769 is an unconjugated rabbit polyclonal antibody to SPHK1 (SPHK) (aa286-315) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
- Application
- IHC, IHC-P, WB
- Reactivity
- Hu, Ms, NHP, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- SK1 | Sphingosine kinase 1 | SPHK | SPHK1 | SK 1 | SPK 1 | Sphingosine kinase type 1 | SPK
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9NYA1
- NCBI GenBank
- NM_021972 | NP_068807.2
- SwissProt
- Q9NYA1
Target
- Target
- Human SPHK / SPHK1
- Antigen Species
- Human
- Epitope
- This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
- Gene Name
- SPHK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 200 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions. Fischer I, Alliod C, Martinier N, Newcombe J, Brana C, Pouly S. PloS one. 2011;6:e23905. PubMed: 21887342 PMC: PMC3161076