Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SORBS1 / Ponsin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120663-100
Catalog Number: LS-G120663
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SORBS1 (NM_001034955), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C-Cbl associated protein | C-Cbl-associated protein | CAP | FLAF2 | KIAA0894 | KIAA1296 | R85FL | SH3 domain protein 5 | SH3-domain protein 5 (ponsin) | SH3P12 | SORB1 | Fas-ligand associated factor 2 | Ponsin | SH3D5 | SORBS1
- Origin Species
- Human
- NCBI GenBank
- NM_015385
- SwissProt
- Q9BX66
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 127.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSECDGGSK AVMNGLAPGS NGQDKATADP LRARSISAVK IIPVKTVKNA SGLVLPTDMD61PTKICTGKGA VTLRASSSYR ETPSSSPASP QETRQHESKP GLEPEPSSAD EWRLSSSADA121NGNAQPSSLA AKGYRSVHPN LPSDKSQDAT SSSAAQPEVI VVPLYLVNTD RGQEGTARPP181TPLGPLGCVP TIPATASAAS PLTFPTLDDF IPPHLQRWPH HSQPARASGS FAPISQTPPS241FSPPPPLVPP APEDLRRVSE PDLTGAVSST DSSPLLNEVS SSLIGTDSQA FPSVSKPSSA301YPSTTIVNPT IVLLQHNREQ QKRLSSLSDP VSERRVGEQD SAPTQEKPTS PGKAIEKRAK361DDSRRVVKST QDLSDVSMDE VGIPLRNTER SKDWYKTMFK QIHKLNRDTP EENPYFPTYK421FPELPEIQQT SEETKSCSVM SPRLECSGTV IAHCSLKLLD SSNPPTSASQ VAGTADDDSD481LYSPRYSFSE DTKSPLSVPR SKSEMSYIDG EKVVKRSATL PLPARSSSLK SSSERNDWEP541PDKKVDTRKY RAEPKSIYEY QPGKSSVLTN EKMSRDISPE EIDLKNEPWY KFFSELEFGK601PPPKKIWDYT PGDCSILPRE DRKTNLDKDL SLCQTELEAD LEKMETLNKA PSANVPQSSA661ISPTPEISSE TPGYIYSSNF HAVKRESDGA PGDLTSLENE RQIYKSVLEG GDIPLQGLSG721LKRPSSSAST KDSESPRHFI PADYLESTEE FIRRRHDDKE KLLADQRRLK REQEEADIAA781RRHTGVIPTH HQFITNERFG DLLNIDDTAK RKSGSEMRPA RAKFDFKAQT LKELPLQKGD841IVYIYKQIDQ NWYEGEHHGR VGIFPRTYIE LLPPAEKAQP KKLTPVQVLE YGEAIAKFNF901NGDTQVEMSF RKGERITLLR QVDENWYEGR IPGTSRQGIF PITYVDVIKR PLVKNPVDYM961DLPFSSSPSR SATASPQPWR EESGQYERKA ERGAGERGPG GPKISKKSCL KPSDVVRCLS1021TEQRLSDLNT PEESRPGKPL GSAFPGSEAE QTERHRGGEQ AGRKAARRGG SQQPQAQQRR1081VTPDRSQTSQ DLFSYQALYS YIPQNDDELE LRDGDIVDVM EKCDDGWFVG TSRRTKQFGT1141FPGNYVKPLY LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1151 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.