LSBio
Human SORBS1 / Ponsin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SORBS1 / Ponsin Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SORBS1 antibodies →
$220.00each
Catalog Number: LS-G87183-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SORBS1 (NM_001034955) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C-Cbl associated protein | C-Cbl-associated protein | CAP | FLAF2 | KIAA0894 | KIAA1296 | R85FL | SH3 domain protein 5 | SH3-domain protein 5 (ponsin) | SH3P12 | SORB1 | Fas-ligand associated factor 2 | Ponsin | SH3D5 | SORBS1
Origin Species
Human
NCBI GenBank
NM_015385
SwissProt
Q9BX66

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
127.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSECDGGSK AVMNGLAPGS NGQDKATADP LRARSISAVK IIPVKTVKNA SGLVLPTDMD
61PTKICTGKGA VTLRASSSYR ETPSSSPASP QETRQHESKP GLEPEPSSAD EWRLSSSADA
121NGNAQPSSLA AKGYRSVHPN LPSDKSQDAT SSSAAQPEVI VVPLYLVNTD RGQEGTARPP
181TPLGPLGCVP TIPATASAAS PLTFPTLDDF IPPHLQRWPH HSQPARASGS FAPISQTPPS
241FSPPPPLVPP APEDLRRVSE PDLTGAVSST DSSPLLNEVS SSLIGTDSQA FPSVSKPSSA
301YPSTTIVNPT IVLLQHNREQ QKRLSSLSDP VSERRVGEQD SAPTQEKPTS PGKAIEKRAK
361DDSRRVVKST QDLSDVSMDE VGIPLRNTER SKDWYKTMFK QIHKLNRDTP EENPYFPTYK
421FPELPEIQQT SEETKSCSVM SPRLECSGTV IAHCSLKLLD SSNPPTSASQ VAGTADDDSD
481LYSPRYSFSE DTKSPLSVPR SKSEMSYIDG EKVVKRSATL PLPARSSSLK SSSERNDWEP
541PDKKVDTRKY RAEPKSIYEY QPGKSSVLTN EKMSRDISPE EIDLKNEPWY KFFSELEFGK
601PPPKKIWDYT PGDCSILPRE DRKTNLDKDL SLCQTELEAD LEKMETLNKA PSANVPQSSA
661ISPTPEISSE TPGYIYSSNF HAVKRESDGA PGDLTSLENE RQIYKSVLEG GDIPLQGLSG
721LKRPSSSAST KDSESPRHFI PADYLESTEE FIRRRHDDKE KLLADQRRLK REQEEADIAA
781RRHTGVIPTH HQFITNERFG DLLNIDDTAK RKSGSEMRPA RAKFDFKAQT LKELPLQKGD
841IVYIYKQIDQ NWYEGEHHGR VGIFPRTYIE LLPPAEKAQP KKLTPVQVLE YGEAIAKFNF
901NGDTQVEMSF RKGERITLLR QVDENWYEGR IPGTSRQGIF PITYVDVIKR PLVKNPVDYM
961DLPFSSSPSR SATASPQPWR EESGQYERKA ERGAGERGPG GPKISKKSCL KPSDVVRCLS
1021TEQRLSDLNT PEESRPGKPL GSAFPGSEAE QTERHRGGEQ AGRKAARRGG SQQPQAQQRR
1081VTPDRSQTSQ DLFSYQALYS YIPQNDDELE LRDGDIVDVM EKCDDGWFVG TSRRTKQFGT
1141FPGNYVKPLY L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1151 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.