Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SORBS1 / Ponsin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115311-100
Catalog Number: LS-G115311
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SORBS1 (NM_006434), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C-Cbl associated protein | C-Cbl-associated protein | CAP | FLAF2 | KIAA0894 | KIAA1296 | R85FL | SH3 domain protein 5 | SH3-domain protein 5 (ponsin) | SH3P12 | SORB1 | Fas-ligand associated factor 2 | Ponsin | SH3D5 | SORBS1
- Origin Species
- Human
- NCBI GenBank
- NM_015385
- SwissProt
- Q9BX66
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSECDGGSK AVMNGLAPGS NGQDKDMDPT KICTGKGAVT LRASSSYRET PSSSPASPQE61TRQHESKPDE WRLSSSADAN GNAQPSSLAA KGYRSVHPNL PSDKSQDSSP LLNEVSSSLI121GTDSQAFPSV SKPSSAYPST TIVNPTIVLL QHNREQQKRL SSLSDPVSER RVGEQDSAPT181QEKPTSPGKA IEKRAKDDSR RVVKSTQDLS DVSMDEVGIP LRNTERSKDW YKTMFKQIHK241LNRDDDSDLY SPRYSFSEDT KSPLSVPRSK SEMSYIDGEK VVKRSATLPL PARSSSLKSS301SERNDWEPPD KKVDTRKYRA EPKSIYEYQP GKSSVLTNEK MSSAISPTPE ISSETPGYIY361SSNFHAVKRE SDGAPGDLTS LENERQIYKS VLEGGDIPLQ GLSGLKRPSS SASTKDSESP421RHFIPADYLE STEEFIRRRH DDKEMRPARA KFDFKAQTLK ELPLQKGDIV YIYKQIDQNW481YEGEHHGRVG IFPRTYIELL PPAEKAQPKK LTPVQVLEYG EAIAKFNFNG DTQVEMSFRK541GERITLLRQV DENWYEGRIP GTSRQGIFPI TYVDVIKRPL VKNPVDYMDL PFSSSPSRSA601TASPQQPQAQ QRRVTPDRSQ TSQDLFSYQA LYSYIPQNDD ELELRDGDIV DVMEKCDDGW661FVGTSRRTKQ FGTFPGNYVK PLYLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
684 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.