Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SGK1 / SGK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126149-100
Catalog Number: LS-G126149
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SGK1 (NM_001143678), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hsgk | Hsgk1 | SGK | SGK1 | Sgk1 variant i3
- Origin Species
- Human
- NCBI GenBank
- NM_005627
- SwissProt
- O00141
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGEMQGALAR ARLESLLRPR HKKRAEAQKR SESFLLSGLA FMKQRRMGLN DFIQKIANNS61YACKHPEVQS ILKISQPQEP ELMNANPSPP PSPSQQINLG PSSNPHAKPS DFHFLKVIGK121GSFGKVLLAR HKAEEVFYAV KVLQKKAILK KKEEKHIMSE RNVLLKNVKH PFLVGLHFSF181QTADKLYFVL DYINGGELFY HLQRERCFLE PRARFYAAEI ASALGYLHSL NIVYRDLKPE241NILLDSQGHI VLTDFGLCKE NIEHNSTTST FCGTPEYLAP EVLHKQPYDR TVDWWCLGAV301LYEMLYGLPP FYSRNTAEMY DNILNKPLQL KPNITNSARH LLEGLLQKDR TKRLGAKDDF361MEIKSHVFFS LINWDDLINK KITPPFNPNV SGPNDLRHFD PEFTEEPVPN SIGKSPDSVL421VTASVKEAAE AFLGFSYAPP TDSFLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
445 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.