LSBio
IHC-plus™ SGK1 / SGK Antibody (aa400-431)

Anti-SGK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

IHC-plus™ SGK1 / SGK Antibody (aa400-431)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$470.00each
Catalog Number: LS-B69-50

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Overview

Description
SGK antibody LS-B69 is an unconjugated rabbit polyclonal antibody to SGK (SGK1) (aa400-431) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Dog, Chicken, Mouse, Non-Human Primate, Rabbit, Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Hsgk | Hsgk1 | SGK | SGK1 | Sgk1 variant i3
Recommended Dilution: ELISA
1:4000 - 1:16000
Recommended Dilution: IHC-P
10 - 20 µg/ml
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
O00141
SwissProt
O00141

Target

Target
SGK1 / SGK
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, macaque and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with SGK-1 homologues from mouse, rat, dog, chicken, bovine, rabbit and spiny dogfish based on an 92% sequence homology for the immunogen (12/13) from these sources. Cross reactivity with SGK-1 homologues from other sources has not been determined.
Epitope
A BLAST analysis was used to suggest reactivity with this protein from human, macaque and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with SGK-1 homologues from mouse, rat, dog, chicken, bovine, rabbit and spiny dogfish based on an 92% sequence homology for the immunogen (12/13) from these sources. Cross reactivity with SGK-1 homologues from other sources has not been determined.
Gene Name
SGK1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
SKG-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 400-431 of human SGK -1.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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