Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SGK1 / SGK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126146-100
Catalog Number: LS-G126146
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SGK1 (NM_001143676), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hsgk | Hsgk1 | SGK | SGK1 | Sgk1 variant i3
- Origin Species
- Human
- NCBI GenBank
- NM_005627
- SwissProt
- O00141
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVNKDMNGFP VKKCSAFQFF KKRVRRWIKS PMVSVDKHQS PSLKYTGSSM VHIPPGEPDF61ESSLCQTCLG EHAFQRGVLP QENESCSWET QSGCEVREPC NHANILTKPD PRTFWTNDDP121AFMKQRRMGL NDFIQKIANN SYACKHPEVQ SILKISQPQE PELMNANPSP PPSPSQQINL181GPSSNPHAKP SDFHFLKVIG KGSFGKVLLA RHKAEEVFYA VKVLQKKAIL KKKEEKHIMS241ERNVLLKNVK HPFLVGLHFS FQTADKLYFV LDYINGGELF YHLQRERCFL EPRARFYAAE301IASALGYLHS LNIVYRDLKP ENILLDSQGH IVLTDFGLCK ENIEHNSTTS TFCGTPEYLA361PEVLHKQPYD RTVDWWCLGA VLYEMLYGLP PFYSRNTAEM YDNILNKPLQ LKPNITNSAR421HLLEGLLQKD RTKRLGAKDD FMEIKSHVFF SLINWDDLIN KKITPPFNPN VSGPNDLRHF481DPEFTEEPVP NSIGKSPDSV LVTASVKEAA EAFLGFSYAP PTDSFLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
526 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.