Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SERPINB4 / SCCA1+2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117634-100
Catalog Number: LS-G117634
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SERPINB4 (NM_002974), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PI-11 | LEUPIN | SCCA2/SCCA1 fusion protein | Serpin B4 | PI11 | SCCA-2 | SCCA1 | Peptidase inhibitor 11 | SCCA2 | SERPINB4
- Origin Species
- Human
- NCBI GenBank
- U19576
- SwissProt
- P48594
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MNSLSEANTK FMFDLFQQFR KSKENNIFYS PISITSALGM VLLGAKDNTA QQISKVLHFD61QVTENTTEKA ATYHVDRSGN VHHQFQKLLT EFNKSTDAYE LKIANKLFGE KTYQFLQEYL121DAIKKFYQTS VESTDFANAP EESRKKINSW VESQTNEKIK NLFPDGTIGN DTTLVLVNAI181YFKGQWENKF KKENTKEEKF WPNKNTYKSV QMMRQYNSFN FALLEDVQAK VLEIPYKGKD241LSMIVLLPNE IDGLQKLEEK LTAEKLMEWT SLQNMRETCV DLHLPRFKME ESYDLKDTLR301TMGMVNIFNG DADLSGMTWS HGLSVSKVLH KAFVEVTEEG VEAAAATAVV VVELSSPSTN361EEFCCNHPFL FFIRQNKTNS ILFYGRFSSPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
390 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.