Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA4C / Semaphorin 4C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101500-100
Catalog Number: LS-G101500
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEMA4C (NM_017789), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- M-Sema F | SEMAF | SEMACL1 | Semaphorin-4C | SEMA4C | KIAA1739 | M-SEMA-F | SEMAI
- Origin Species
- Human
- NCBI GenBank
- NM_017789
- SwissProt
- Q9C0C4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPHWAVWLL AARLWGLGIG AEVWWNLVPR KTVSSGELAT VVRRFSQTGI QDFLTLTLTE61PTGLLYVGAR EALFAFSMEA LELQGAISWE APVEKKTECI QKGKNNQTEC FNFIRFLQPY121NASHLYVCGT YAFQPKCTYV NMLTFTLEHG EFEDGKGKCP YDPAKGHAGL LVDGELYSAT181LNNFLGTEPI ILRNMGPHHS MKTEYLAFWL NEPHFVGSAY VPESVGSFTG DDDKVYFFFR241ERAVESDCYA EQVVARVARV CKGDMGGART LQRKWTTFLK ARLACSAPNW QLYFNQLQAM301HTLQDTSWHN TTFFGVFQAQ WGDMYLSAIC EYQLEEIQRV FEGPYKEYHE EAQKWDRYTD361PVPSPRPGSC INNWHRRHGY TSSLELPDNI LNFVKKHPLM EEQVGPRWSR PLLVKKGTNF421THLVADRVTG LDGATYTVLF IGTGDGWLLK AVSLGPWVHL IEELQLFDQE PMRSLVLSQS481KKLLFAGSRS QLVQLPVADC MKYRSCADCV LARDPYCAWS VNTSRCVAVG GHSGSLLIQH541VMTSDTSGIC NLRGSKKVRP TPKNITVVAG TDLVLPCHLS SNLAHARWTF GGRDLPAEQP601GSFLYDARLQ ALVVMAAQPR HAGAYHCFSE EQGARLAAEG YLVAVVAGPS VTLEARAPLE661NLGLVWLAVV ALGAVCLVLL LLVLSLRRRL REELEKGAKA TERTLVYPLE LPKEPTSPPF721RPCPEPDEKL WDPVGYYYSD GSLKIVPGHA RCQPGGGPPS PPPGIPGQPL PSPTRLHLGG781GRNSNANGYV RLQLGGEDRG GLGHPLPELA DELRRKLQQR QPLPDSNPEE SSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
833 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.