SEMA4C / Semaphorin 4C Antibody
Catalog Number: LS-C409760
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SEMA4C / Semaphorin 4C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G82254-20
Catalog Number: LS-G82254
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SEMA4C (NM_017789) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- M-Sema F | SEMAF | SEMACL1 | Semaphorin-4C | SEMA4C | KIAA1739 | M-SEMA-F | SEMAI
- Origin Species
- Human
- NCBI GenBank
- NM_017789
- SwissProt
- Q9C0C4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPHWAVWLL AARLWGLGIG AEVWWNLVPR KTVSSGELAT VVRRFSQTGI QDFLTLTLTE61PTGLLYVGAR EALFAFSMEA LELQGAISWE APVEKKTECI QKGKNNQTEC FNFIRFLQPY121NASHLYVCGT YAFQPKCTYV NMLTFTLEHG EFEDGKGKCP YDPAKGHAGL LVDGELYSAT181LNNFLGTEPI ILRNMGPHHS MKTEYLAFWL NEPHFVGSAY VPESVGSFTG DDDKVYFFFR241ERAVESDCYA EQVVARVARV CKGDMGGART LQRKWTTFLK ARLACSAPNW QLYFNQLQAM301HTLQDTSWHN TTFFGVFQAQ WGDMYLSAIC EYQLEEIQRV FEGPYKEYHE EAQKWDRYTD361PVPSPRPGSC INNWHRRHGY TSSLELPDNI LNFVKKHPLM EEQVGPRWSR PLLVKKGTNF421THLVADRVTG LDGATYTVLF IGTGDGWLLK AVSLGPWVHL IEELQLFDQE PMRSLVLSQS481KKLLFAGSRS QLVQLPVADC MKYRSCADCV LARDPYCAWS VNTSRCVAVG GHSGSLLIQH541VMTSDTSGIC NLRGSKKVRP TPKNITVVAG TDLVLPCHLS SNLAHARWTF GGRDLPAEQP601GSFLYDARLQ ALVVMAAQPR HAGAYHCFSE EQGARLAAEG YLVAVVAGPS VTLEARAPLE661NLGLVWLAVV ALGAVCLVLL LLVLSLRRRL REELEKGAKA TERTLVYPLE LPKEPTSPPF721RPCPEPDEKL WDPVGYYYSD GSLKIVPGHA RCQPGGGPPS PPPGIPGQPL PSPTRLHLGG781GRNSNANGYV RLQLGGEDRG GLGHPLPELA DELRRKLQQR QPLPDSNPEE SSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
833 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
