Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SCRN1 / Secernin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126746-100
Catalog Number: LS-G126746
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCRN1 (NM_001145514), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0193 | Secernin-1 | SES1 | SCRN1 | Secernin 1
- Origin Species
- Human
- NCBI GenBank
- NM_014766
- SwissProt
- Q12765
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVQDGTFKTR DSTWTCESTR MAAAPPSYCF VAFPPRAKDG LVVFGKNSAR PRDEVQEVVY61FSAADHEPES KVECTYISID QVPRTYAIMI SRPAWLWGAE MGANEHGVCI ANEAINTREP121AAEIEALLGM DLVRLGLERG ETAKEALDVI VSLLEEHGQG GNYFEDANSC HSFQSAYLIV181DRDEAWVLET IGKYWAAEKV TEGVRCICSQ LSLTTKMDAE HPELRSYAQS QGWWTGEGEF241NFSEVFSPVE DHLDCGAGKD SLEKQEESIT VQTMMNTLRD KASGVCIDSE FFLTTASGVS301VLPQNRSSPC IHYFTGTPDP SRSIFKPFIF VDDVKLVPKT QSPCFGDDDP AKKEPRFQEK361PDRRHELYKA HEWARAIIES DQEQGRKLRS TMLELEKQGL EAMEEILTSS EPLDPAEVGD421LFYDCVDTEI KFFKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
434 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.