Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SCRN1 / Secernin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113661-100
Catalog Number: LS-G113661
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCRN1 (NM_014766), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0193 | Secernin-1 | SES1 | SCRN1 | Secernin 1
- Origin Species
- Human
- NCBI GenBank
- NM_014766
- SwissProt
- Q12765
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAPPSYCF VAFPPRAKDG LVVFGKNSAR PRDEVQEVVY FSAADHEPES KVECTYISID61QVPRTYAIMI SRPAWLWGAE MGANEHGVCI ANEAINTREP AAEIEALLGM DLVRLGLERG121ETAKEALDVI VSLLEEHGQG GNYFEDANSC HSFQSAYLIV DRDEAWVLET IGKYWAAEKV181TEGVRCICSQ LSLTTKMDAE HPELRSYAQS QGWWTGEGEF NFSEVFSPVE DHLDCGAGKD241SLEKQEESIT VQTMMNTLRD KASGVCIDSE FFLTTASGVS VLPQNRSSPC IHYFTGTHDP301SRSIFKPFIF VDDVKLVPKT QSPCFGDDDP AKKEPRFQEK PDRRHELYKA HEWARAIIES361DQEQGRKLRS TMLELEKQGL EAMEEILTSS EPLDPAEVGD LFYDCVDTEI KFFKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
414 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.