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ROBO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ROBO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ROBO1 antibodies →
$725.00each
Catalog Number: LS-G127329-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ROBO1 (NM_133631), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deleted in U twenty twenty | H-Robo-1 | Roundabout 1 | SAX3 | DUTT1 | ROBO1 | Roundabout homolog 1
Origin Species
Human
NCBI GenBank
NM_002941
SwissProt
Q9Y6N7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
175.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC KAEGRPTPTI
61EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR PDEGVYVCVA RNYLGEAVSH
121NASLEVAILR DDFRQNPSDV MVAVGEPAVM ECQPPRGHPE PTISWKKDGS PLDDKDERIT
181IRGGKLMITY TRKSDAGKYV CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA
241EFKCEARGDP VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA
301EASATLTVQV GSEPPHFVVK PRDQVVALGR TVTFQCEATG NPQPAIFWRR EGSQNLLFSY
361QPPQSSSRFS VSQTGDLTIT NVQRSDVGYY ICQTLNVAGS IITKAYLEVT DVIADRPPPV
421IRQGPVNQTV AVDGTFVLSC VATGSPVPTI LWRKDGVLVS TQDSRIKQLE NGVLQIRYAK
481LGDTGRYTCI ASTPSGEATW SAYIEVQEFG VPVQPPRPTD PNLIPSAPSK PEVTDVSRNT
541VTLSWQPNLN SGATPTSYII EAFSHASGSS WQTVAENVKT ETSAIKGLKP NAIYLFLVRA
601ANAYGISDPS QISDPVKTQD VLPTSQGVDH KQVQRELGNA VLHLHNPTVL SSSSIEVHWT
661VDQQSQYIQG YKILYRPSGA NHGESDWLVF EVRTPAKNSV VIPDLRKGVN YEIKARPFFN
721EFQGADSEIK FAKTLEEAPS APPQGVTVSK NDGNGTAILV SWQPPPEDTQ NGMVQEYKVW
781CLGNETRYHI NKTVDGSTFS VVIPFLVPGI RYSVEVAAST GAGSGVKSEP QFIQLDAHGN
841PVSPEDQVSL AQQISDVVKQ PAFIAGIGAA CWIILMVFSI WLYRHRKKRN GLTSTYAGIR
901KVPSFTFTPT VTYQRGGEAV SSGGRPGLLN ISEPAAQPWL ADTWPNTGNN HNDCSISCCT
961AGNGNSDSNL TTYSRPADCI ANYNNQLDNK QTNLMLPEST VYGDVDLSNK INEMKTFNSP
1021NLKDGRFVNP SGQPTPYATT QLIQSNLSNN MNNGSGDSGE KHWKPLGQQK QEVAPVQYNI
1081VEQNKLNKDY RANDTVPPTI PYNQSYDQNT GGSYNSSDRG SSTSGSQGHK KGARTPKVPK
1141QGGMNWADLL PPPPAHPPPH SNSEEYNISV DESYDQEMPC PVPPARMYLQ QDELEEEEDE
1201RGPTPPVRGA ASSPAAVSYS HQSTATLTPS PQEELQPMLQ DCPEETGHMQ HQPDRRRQPV
1261SPPPPPRPIS PPHTYGYISG PLVSDMDTDA PEEEEDEADM EVAKMQTRRL LLRGLEQTPA
1321SSVGDLESSV TGSMINGWGS ASEEDNISSG RSSVSSSDGS FFTDADFAQA VAAAAEYAGL
1381KVARRQMQDA AGRRHFHASQ CPRPTSPVST DSNMSAAVMQ KTRPAKKLKH QPGHLRRETY
1441TDDLPPPPVP PPAIKSPTAQ SKTQLEVRPV VVPKLPSMDA RTDRSSDRKG SSYKGREVLD
1501GRQVVDMRTN PGDPREAQEQ QNDGKGRGNK AAKRDLPPAK THLIQEDILP YCRPTFPTSN
1561NPRDPSSSSS MSSRGSGSRQ REQANVGRRN IAEMQVLGGY ERGEDNNEEL EETES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1615 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.