ROBO1 Antibody
Catalog Number: LS-C750283
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ROBO1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G88800-20
Catalog Number: LS-G88800
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ROBO1 (NM_133631) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Deleted in U twenty twenty | H-Robo-1 | Roundabout 1 | SAX3 | DUTT1 | ROBO1 | Roundabout homolog 1
- Origin Species
- Human
- NCBI GenBank
- NM_002941
- SwissProt
- Q9Y6N7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 175.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC KAEGRPTPTI61EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR PDEGVYVCVA RNYLGEAVSH121NASLEVAILR DDFRQNPSDV MVAVGEPAVM ECQPPRGHPE PTISWKKDGS PLDDKDERIT181IRGGKLMITY TRKSDAGKYV CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA241EFKCEARGDP VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA301EASATLTVQV GSEPPHFVVK PRDQVVALGR TVTFQCEATG NPQPAIFWRR EGSQNLLFSY361QPPQSSSRFS VSQTGDLTIT NVQRSDVGYY ICQTLNVAGS IITKAYLEVT DVIADRPPPV421IRQGPVNQTV AVDGTFVLSC VATGSPVPTI LWRKDGVLVS TQDSRIKQLE NGVLQIRYAK481LGDTGRYTCI ASTPSGEATW SAYIEVQEFG VPVQPPRPTD PNLIPSAPSK PEVTDVSRNT541VTLSWQPNLN SGATPTSYII EAFSHASGSS WQTVAENVKT ETSAIKGLKP NAIYLFLVRA601ANAYGISDPS QISDPVKTQD VLPTSQGVDH KQVQRELGNA VLHLHNPTVL SSSSIEVHWT661VDQQSQYIQG YKILYRPSGA NHGESDWLVF EVRTPAKNSV VIPDLRKGVN YEIKARPFFN721EFQGADSEIK FAKTLEEAPS APPQGVTVSK NDGNGTAILV SWQPPPEDTQ NGMVQEYKVW781CLGNETRYHI NKTVDGSTFS VVIPFLVPGI RYSVEVAAST GAGSGVKSEP QFIQLDAHGN841PVSPEDQVSL AQQISDVVKQ PAFIAGIGAA CWIILMVFSI WLYRHRKKRN GLTSTYAGIR901KVPSFTFTPT VTYQRGGEAV SSGGRPGLLN ISEPAAQPWL ADTWPNTGNN HNDCSISCCT961AGNGNSDSNL TTYSRPADCI ANYNNQLDNK QTNLMLPEST VYGDVDLSNK INEMKTFNSP1021NLKDGRFVNP SGQPTPYATT QLIQSNLSNN MNNGSGDSGE KHWKPLGQQK QEVAPVQYNI1081VEQNKLNKDY RANDTVPPTI PYNQSYDQNT GGSYNSSDRG SSTSGSQGHK KGARTPKVPK1141QGGMNWADLL PPPPAHPPPH SNSEEYNISV DESYDQEMPC PVPPARMYLQ QDELEEEEDE1201RGPTPPVRGA ASSPAAVSYS HQSTATLTPS PQEELQPMLQ DCPEETGHMQ HQPDRRRQPV1261SPPPPPRPIS PPHTYGYISG PLVSDMDTDA PEEEEDEADM EVAKMQTRRL LLRGLEQTPA1321SSVGDLESSV TGSMINGWGS ASEEDNISSG RSSVSSSDGS FFTDADFAQA VAAAAEYAGL1381KVARRQMQDA AGRRHFHASQ CPRPTSPVST DSNMSAAVMQ KTRPAKKLKH QPGHLRRETY1441TDDLPPPPVP PPAIKSPTAQ SKTQLEVRPV VVPKLPSMDA RTDRSSDRKG SSYKGREVLD1501GRQVVDMRTN PGDPREAQEQ QNDGKGRGNK AAKRDLPPAK THLIQEDILP YCRPTFPTSN1561NPRDPSSSSS MSSRGSGSRQ REQANVGRRN IAEMQVLGGY ERGEDNNEEL EETESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1615 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
