Anti-ROBO1 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ ROBO1 Antibody (aa1625-1650)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$526.00each

Catalog Number: LS-B81-50

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Overview

Description: ROBO1 antibody LS-B81 is an unconjugated rabbit polyclonal antibody to ROBO1 (aa1625-1650) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application: ELISA, IF, IHC, IHC-P, IP, WB
Reactivity: Hu
Predicted Reactivity: Dog, Mouse, Rat
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Deleted in U twenty twenty | H-Robo-1 | Roundabout 1 | SAX3 | DUTT1 | ROBO1 | Roundabout homolog 1
Recommended Dilution: ELISA: 1:30000 - 1:60000
Recommended Dilution: IHC-P: 2.5 µg/ml
Recommended Dilution: WB: 1:500 - 1:3000
UniProt Number: Q9Y6N7
NCBI GenBank: NM_002941 | NP_002932.1
SwissProt: Q9Y6N7

Target

Target: ROBO1
Antigen Species: Human
Specificity: A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of ROBO-1. Cross reactivity with ROBO-1 homologues from other sources has not been determined.
Epitope: A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of ROBO-1. Cross reactivity with ROBO-1 homologues from other sources has not been determined.
Gene Name: ROBO1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1.1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl