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RASGRP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RASGRP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RASGRP4 antibodies →
$504.00each
Catalog Number: LS-G128270-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASGRP 4(RASGRP4) (NM_001146204), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RAS guanyl releasing protein 4 | RASGRP 4 | RAS guanyl-releasing protein 4 | RASGRP4
Origin Species
Human
NCBI GenBank
NM_001146205
SwissProt
Q8TDF6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNRKDSKRKS HQECTGKIGG RGRPRQVRRH KTCPSPREIS KVMASMNLGL LSEGGCSEDE
61LLEKCIQSFD SAGSLCHEDH MLNMVLAMHS WVLPSADLAA RLLTSYQKAT GDTQELRRLQ
121ICHLVRYWLM RHPEVMHQDP QLEEVIGRFW ATVAREGNSA QRRLGDSSDL LSPGGPGPPL
181PMSSPGLGKK RKVSLLFDHL ETGELAQHLT YLEFRSFQAI TPQDLRSYVL QGSVRGCPAL
241EGSVGLSNSV SRWVQVMVLS RPGPLQRAQV LDKFIHVAQR LHQLQNFNTL MAVTGGLCHS
301AISRLKDSHA HLSPDSTKEL VALQGQHPPC SANEDLLHLL TLSLDLFYTE DEIYELSYAR
361EPRCPKSLPP SPFNAPLVVE WAPGVTPKPD RVTLGRHVEQ LVESVFKNYD PEGRGTISQE
421DFERLSGNFP FACHGLHPPP RQGRGSFSRE ELTGYLLRAS AICSKLGLAF LHTFHEVTFR
481KPTFCDSCSG FLWGVTKQGY RCRECGLCCH KHCRDQVKVE CKKRPGAKGD AGPPGAPVPS
541TPAPHASCGS EENHSYTLSL EPETGCQLRH AWTQTESPHP SWETDTVPCP VMDPPSTASS
601KLDS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

604 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.