Human RASGRP4 Protein (Recombinant GST)
Catalog Number: LS-G36228
2 sizes / from $489.00
Expression System Tobacco / Plant-Based Expression
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RASGRP4 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93232-20
Catalog Number: LS-G93232
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RASGRP 4(RASGRP4) (NM_001146204) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RAS guanyl releasing protein 4 | RASGRP 4 | RAS guanyl-releasing protein 4 | RASGRP4
- Origin Species
- Human
- NCBI GenBank
- NM_001146205
- SwissProt
- Q8TDF6
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNRKDSKRKS HQECTGKIGG RGRPRQVRRH KTCPSPREIS KVMASMNLGL LSEGGCSEDE61LLEKCIQSFD SAGSLCHEDH MLNMVLAMHS WVLPSADLAA RLLTSYQKAT GDTQELRRLQ121ICHLVRYWLM RHPEVMHQDP QLEEVIGRFW ATVAREGNSA QRRLGDSSDL LSPGGPGPPL181PMSSPGLGKK RKVSLLFDHL ETGELAQHLT YLEFRSFQAI TPQDLRSYVL QGSVRGCPAL241EGSVGLSNSV SRWVQVMVLS RPGPLQRAQV LDKFIHVAQR LHQLQNFNTL MAVTGGLCHS301AISRLKDSHA HLSPDSTKEL VALQGQHPPC SANEDLLHLL TLSLDLFYTE DEIYELSYAR361EPRCPKSLPP SPFNAPLVVE WAPGVTPKPD RVTLGRHVEQ LVESVFKNYD PEGRGTISQE421DFERLSGNFP FACHGLHPPP RQGRGSFSRE ELTGYLLRAS AICSKLGLAF LHTFHEVTFR481KPTFCDSCSG FLWGVTKQGY RCRECGLCCH KHCRDQVKVE CKKRPGAKGD AGPPGAPVPS541TPAPHASCGS EENHSYTLSL EPETGCQLRH AWTQTESPHP SWETDTVPCP VMDPPSTASS601KLDSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
604 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
