Human RASGRP4 Protein (Recombinant GST)
Catalog Number: LS-G36228
2 sizes / from $489.00
Expression System Tobacco / Plant-Based Expression
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RASGRP4 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93235-20
Catalog Number: LS-G93235
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RASGRP 4(RASGRP4) (NM_001146207) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RAS guanyl releasing protein 4 | RASGRP 4 | RAS guanyl-releasing protein 4 | RASGRP4
- Origin Species
- Human
- NCBI GenBank
- NM_001146205
- SwissProt
- Q8TDF6
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNRKDSKRKS HQECTGKIGG RGRPRQVRRH KTCPSPREIS KVMASMNLGL LSEGGCSEDE61LLEKCIQSFD SAGSLCHEDH MLNMVLAMHS WVLPSADLAA RLLTSYQKAT GDTQELRRLQ121ICHLVRYWLM RHPEVMHQDP QLEEVIGRFW ATVAREGNSA QRRLGDSSDL LSPGGPGPPL181PMSSPGLGKK RKVSLLFDHL ETGELAQHLT YLEFRSFQAI TALLELTELL ASHNNYARYR241RTWAGCAGFR LPVLGVHLKD LVSLHEAQPD RLPDGRLHLP KLNNLYLRLQ ELVALQGQHP301PCSANEDLLH LLTLSLDLFY TEDEIYELSY AREPRCPKSL PPSPFNAPLV VEWAPGVTPK361PDRVTLGRHV EQLVESVFKN YDPEGRGTIS QEDFERLSGN FPFACHGLHP PPRQGRGSFS421REELTGYLLR ASAICSKLGL AFLHTFHEVT FRKPTFCDSC SGFLWGVTKQ GYRCRECGLC481CHKHCRDQVK VECKKRPGAK GDAGPPGAPV PSTPAPHASC GSEENHSYTL SLEPETGCQL541RHAWTQTESP HPSWETDTVP CPVMDPPSTA SSKLDSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
576 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
