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RALGDS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RALGDS Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RALGDS antibodies →
$725.00each
Catalog Number: LS-G119467-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RALGDS (NM_001042368), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1308 | RGDS | RALGDS | RalGEF | RGF
Origin Species
Human
NCBI GenBank
U14417
SwissProt
Q12967

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMVDCQSSTQ EIGEELINGV IYSISLRKVQ LHHGGNKGQR WLGYENESAL NLYETCKVRT
61VKAGTLEKLV EHLVPAFQGS DLSYVTIFLC TYRAFTTTQQ VLDLLFKRYG RCDALTASSR
121YGCILPYSDE DGGPQDQLKN AISSILGTWL DQYSEDFCQP PDFPCLKQLV AYVQLNMPGS
181DLERRAHLLL AQLEHSEPIE AEPEALSPVP ALKPTPELEL ALTPARAPSP VPAPAPEPEP
241APTPAPGSEL EVAPAPAPEL QQAPEPAVGL ESAPAPALEL EPAPEQDPAP SQTLELEPAP
301APVPSLQPSW PSPVVAENGL SEEKPHLLVF PPDLVAEQFT LMDAELFKKV VPYHCLGSIW
361SQRDKKGKEH LAPTIRATVT QFNSVANCVI TTCLGNRSTK APDRARVVEH WIEVARECRI
421LKNFSSLYAI LSALQSNSIH RLKKTWEDVS RDSFRIFQKL SEIFSDENNY SLSRELLIKE
481GTSKFATLEM NPKRAQKRPK ETGIIQGTVP YLGTFLTDLV MLDTAMKDYL YGRLINFEKR
541RKEFEVIAQI KLLQSACNNY SIAPDEQFGA WFRAVERLSE TESYNLSCEL EPPSESASNT
601LRTKKNTAIV KRWSDRQAPS TELSTSGSSH SKSCDQLRCG PYLSSGDIAD ALSVHSAGSS
661SSDVEEINIS FVPESPDGQE KKFWESASQS SPETSGISSA SSSTSSSSAS TTPVAATRTH
721KRSVSGLCNS SSALPLYNQQ VGDCCIIRVS LDVDNGNMYK SILVTSQDKA PAVIRKAMDK
781HNLEEEEPED YELLQILSDD RKLKIPENAN VFYAMNSTAN YDFVLKKRTF TKGVKVKHGA
841SSTLPRMKQK GLKIAKGIF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

859 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.