RALGDS Antibody (aa531-559, APC)
Catalog Number: LS-C217127-200
200 µL / $972.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RALGDS Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87907-20
Catalog Number: LS-G87907
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RALGDS (NM_001042368) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1308 | RGDS | RALGDS | RalGEF | RGF
- Origin Species
- Human
- NCBI GenBank
- U14417
- SwissProt
- Q12967
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 94.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMVDCQSSTQ EIGEELINGV IYSISLRKVQ LHHGGNKGQR WLGYENESAL NLYETCKVRT61VKAGTLEKLV EHLVPAFQGS DLSYVTIFLC TYRAFTTTQQ VLDLLFKRYG RCDALTASSR121YGCILPYSDE DGGPQDQLKN AISSILGTWL DQYSEDFCQP PDFPCLKQLV AYVQLNMPGS181DLERRAHLLL AQLEHSEPIE AEPEALSPVP ALKPTPELEL ALTPARAPSP VPAPAPEPEP241APTPAPGSEL EVAPAPAPEL QQAPEPAVGL ESAPAPALEL EPAPEQDPAP SQTLELEPAP301APVPSLQPSW PSPVVAENGL SEEKPHLLVF PPDLVAEQFT LMDAELFKKV VPYHCLGSIW361SQRDKKGKEH LAPTIRATVT QFNSVANCVI TTCLGNRSTK APDRARVVEH WIEVARECRI421LKNFSSLYAI LSALQSNSIH RLKKTWEDVS RDSFRIFQKL SEIFSDENNY SLSRELLIKE481GTSKFATLEM NPKRAQKRPK ETGIIQGTVP YLGTFLTDLV MLDTAMKDYL YGRLINFEKR541RKEFEVIAQI KLLQSACNNY SIAPDEQFGA WFRAVERLSE TESYNLSCEL EPPSESASNT601LRTKKNTAIV KRWSDRQAPS TELSTSGSSH SKSCDQLRCG PYLSSGDIAD ALSVHSAGSS661SSDVEEINIS FVPESPDGQE KKFWESASQS SPETSGISSA SSSTSSSSAS TTPVAATRTH721KRSVSGLCNS SSALPLYNQQ VGDCCIIRVS LDVDNGNMYK SILVTSQDKA PAVIRKAMDK781HNLEEEEPED YELLQILSDD RKLKIPENAN VFYAMNSTAN YDFVLKKRTF TKGVKVKHGA841SSTLPRMKQK GLKIAKGIFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
859 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
