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RALGDS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RALGDS Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RALGDS antibodies →
$504.00each
Catalog Number: LS-G115421-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RALGDS (NM_006266), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1308 | RGDS | RALGDS | RalGEF | RGF
Origin Species
Human
NCBI GenBank
U14417
SwissProt
Q12967

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVQRMWAEAA GPAGGAEPLF PGSRRSRSVW DAVRLEVGVP DSCPVVLHSF TQLDPDLPRP
61ESSTQEIGEE LINGVIYSIS LRKVQLHHGG NKGQRWLGYE NESALNLYET CKVRTVKAGT
121LEKLVEHLVP AFQGSDLSYV TIFLCTYRAF TTTQQVLDLL FKRYGRCDAL TASSRYGCIL
181PYSDEDGGPQ DQLKNAISSI LGTWLDQYSE DFCQPPDFPC LKQLVAYVQL NMPGSDLERR
241AHLLLAQLEH SEPIEAEPEA LSPVPALKPT PELELALTPA RAPSPVPAPA PEPEPAPTPA
301PGSELEVAPA PAPELQQAPE PAVGLESAPA PALELEPAPE QDPAPSQTLE LEPAPAPVPS
361LQPSWPSPVV AENGLSEEKP HLLVFPPDLV AEQFTLMDAE LFKKVVPYHC LGSIWSQRDK
421KGKEHLAPTI RATVTQFNSV ANCVITTCLG NRSTKAPDRA RVVEHWIEVA RECRILKNFS
481SLYAILSALQ SNSIHRLKKT WEDVSRDSFR IFQKLSEIFS DENNYSLSRE LLIKEGTSKF
541ATLEMNPKRA QKRPKETGII QGTVPYLGTF LTDLVMLDTA MKDYLYGRLI NFEKRRKEFE
601VIAQIKLLQS ACNNYSIAPD EQFGAWFRAV ERLSETESYN LSCELEPPSE SASNTLRTKK
661NTAIVKRWSD RQAPSTELST SGSSHSKSCD QLRCGPYLSS GDIADALSVH SAGSSSSDVE
721EINISFVPES PDGQEKKFWE SASQSSPETS GISSASSSTS SSSASTTPVA ATRTHKRSVS
781GLCNSSSALP LYNQQVGDCC IIRVSLDVDN GNMYKSILVT SQDKAPAVIR KAMDKHNLEE
841EEPEDYELLQ ILSDDRKLKI PENANVFYAM NSTANYDFVL KKRTFTKGVK VKHGASSTLP
901RMKQKGLKIA KGIF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

914 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.