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PTPRS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTPRS Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTPRS antibodies →
$725.00each
Catalog Number: LS-G107587-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPRS (NM_130855), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Bptp-1 | Cryp-alpha | LAR-PTP2 | PTP NE-3 | PTP-varsigma | PTPSIGMA | R-PTP-S | PTP sigma | PTPRS | R-PTP-sigma
Origin Species
Human
NCBI GenBank
NM_002850
SwissProt
Q13332

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
166.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPTWGPGMV SVVGPMGLLV VLLVGGCAAE EPPRFIKEPK DQIGVSGGVA SFVCQATGDP
61KPRVTWNKKG KKVNSQRFET IEFDESAGAV LRIQPLRTPR DENVYECVAQ NSVGEITVHA
121KLTVLREDQL PSGFPNIDMG PQLKVVERTR TATMLCAASG NPDPEITWFK DFLPVDPSAS
181NGRIKQLRSG ALQIESSEET DQGKYECVAT NSAGVRYSSP ANLYVRELRE VRRVAPRFSI
241LPMSHEIMPG GNVNITCVAV GSPMPYVKWM QGAEDLTPED DMPVGRNVLE LTDVKDSANY
301TCVAMSSLGV IEAVAQITVK SLPKAPGTPM VTENTATSIT ITWDSGNPDP VSYYVIEYKS
361KSQDGPYQIK EDITTTRYSI GGLSPNSEYE IWVSAVNSIG QGPPSESVVT RTGEQAPASA
421PRNVQARMLS ATTMIVQWEE PVEPNGLIRG YRVYYTMEPE HPVGNWQKHN VDDSLLTTVG
481SLLEDETYTV RVLAFTSVGD GPLSDPIQVK TQQGVPGQPM NLRAEARSET SITLSWSPPR
541QESIIKYELL FREGDHGREV GRTFDPTTSY VVEDLKPNTE YAFRLAARSP QGLGAFTPVV
601RQRTLQSISP KNFKVKMIMK TSVLLSWEFP DNYNSPTPYK IQYNGLTLDV DGRTTKKLIT
661HLKPHTFYNF VLTNRGSSLG GLQQTVTAWT AFNLLNGKPS VAPKPDADGF IMVYLPDGQS
721PVPVQSYFIV MVPLRKSRGG QFLTPLGSPE DMDLEELIQD ISRLQRRSLR HSRQLEVPRP
781YIAARFSVLP PTFHPGDQKQ YGGFDNRGLE PGHRYVLFVL AVLQKSEPTF AASPFSDPFQ
841LDNPDPQPIV DGEEGLIWVI GPVLAVVFII CIVIAILLYK NKPDSKRKDS EPRTKCLLNN
901ADLAPHHPKD PVEMRRINFQ TPGMLSHPPI PIADMAEHTE RLKANDSLKL SQEYESIDPG
961QQFTWEHSNL EVNKPKNRYA NVIAYDHSRV ILQPIEGIMG SDYINANYVD GYRCQNAYIA
1021TQGPLPETFG DFWRMVWEQR SATIVMMTRL EEKSRIKCDQ YWPNRGTETY GFIQVTLLDT
1081IELATFCVRT FSLHKNGSSE KREVRQFQFT AWPDHGVPEY PTPFLAFLRR VKTCNPPDAG
1141PIVVHCSAGV GRTGCFIVID AMLERIKPEK TVDVYGHVTL MRSQRNYMVQ TEDQYSFIHE
1201ALLEAVGCGN TEVPARSLYA YIQKLAQVEP GEHVTGMELE FKRLANSKAH TSRFISANLP
1261CNKFKNRLVN IMPYESTRVC LQPIRGVEGS DYINASFIDG YRQQKAYIAT QGPLAETTED
1321FWRMLWENNS TIVVMLTKLR EMGREKCHQY WPAERSARYQ YFVVDPMAEY NMPQYILREF
1381KVTDARDGQS RTVRQFQFTD WPEQGVPKSG EGFIDFIGQV HKTKEQFGQD GPISVHCSAG
1441VGRTGVFITL SIVLERMRYE GVVDIFQTVK MLRTQRPAMV QTEDEYQFCY QAALEYLGSF
1501DHYAT
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1505 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.