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PTPRS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTPRS Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTPRS antibodies →
$504.00each
Catalog Number: LS-G107489-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPRS (NM_130853), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Bptp-1 | Cryp-alpha | LAR-PTP2 | PTP NE-3 | PTP-varsigma | PTPSIGMA | R-PTP-S | PTP sigma | PTPRS | R-PTP-sigma
Origin Species
Human
NCBI GenBank
NM_002850
SwissProt
Q13332

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
165.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPTWGPGMV SVVGPMGLLV VLLVGGCAAE EPPRFIKEPK DQIGVSGGVA SFVCQATGDP
61KPRVTWNKKG KKVNSQRFET IEFDESAGAV LRIQPLRTPR DENVYECVAQ NSVGEITVHA
121KLTVLREDQL PSGFPNIDMG PQLKVVERTR TATMLCAASG NPDPEITWFK DFLPVDPSAS
181NGRIKQLRSG ALQIESSEET DQGKYECVAT NSAGVRYSSP ANLYVRVRRV APRFSILPMS
241HEIMPGGNVN ITCVAVGSPM PYVKWMQGAE DLTPEDDMPV GRNVLELTDV KDSANYTCVA
301MSSLGVIEAV AQITVKSLPK APGTPMVTEN TATSITITWD SGNPDPVSYY VIEYKSKSQD
361GPYQIKEDIT TTRYSIGGLS PNSEYEIWVS AVNSIGQGPP SESVVTRTGE QAPASAPRNV
421QARMLSATTM IVQWEEPVEP NGLIRGYRVY YTMEPEHPVG NWQKHNVDDS LLTTVGSLLE
481DETYTVRVLA FTSVGDGPLS DPIQVKTQQG VPGQPMNLRA EARSETSITL SWSPPRQESI
541IKYELLFREG DHGREVGRTF DPTTSYVVED LKPNTEYAFR LAARSPQGLG AFTPVVRQRT
601LQSISPKNFK VKMIMKTSVL LSWEFPDNYN SPTPYKIQYN GLTLDVDGRT TKKLITHLKP
661HTFYNFVLTN RGSSLGGLQQ TVTAWTAFNL LNGKPSVAPK PDADGFIMVY LPDGQSPVPV
721QSYFIVMVPL RKSRGGQFLT PLGSPEDMDL EELIQDISRL QRRSLRHSRQ LEVPRPYIAA
781RFSVLPPTFH PGDQKQYGGF DNRGLEPGHR YVLFVLAVLQ KSEPTFAASP FSDPFQLDNP
841DPQPIVDGEE GLIWVIGPVL AVVFIICIVI AILLYKNKPD SKRKDSEPRT KCLLNNADLA
901PHHPKDPVEM RRINFQTPGM LSHPPIPIAD MAEHTERLKA NDSLKLSQEY ESIDPGQQFT
961WEHSNLEVNK PKNRYANVIA YDHSRVILQP IEGIMGSDYI NANYVDGYRR QNAYIATQGP
1021LPETFGDFWR MVWEQRSATI VMMTRLEEKS RIKCDQYWPN RGTETYGFIQ VTLLDTIELA
1081TFCVRTFSLH KNGSSEKREV RQFQFTAWPD HGVPEYPTPF LAFLRRVKTC NPPDAGPIVV
1141HCSAGVGRTG CFIVIDAMLE RIKPEKTVDV YGHVTLMRSQ RNYMVQTEDQ YSFIHEALLE
1201AVGCGNTEVP ARSLYAYIQK LAQVEPGEHV TGMELEFKRL ANSKAHTSRF ISANLPCNKF
1261KNRLVNIMPY ESTRVCLQPI RGVEGSDYIN ASFIDGYRQQ KAYIATQGPL AETTEDFWRM
1321LWENNSTIVV MLTKLREMGR EKCHQYWPAE RSARYQYFVV DPMAEYNMPQ YILREFKVTD
1381ARDGQSRTVR QFQFTDWPEQ GVPKSGEGFI DFIGQVHKTK EQFGQDGPIS VHCSAGVGRT
1441GVFITLSIVL ERMRYEGVVD IFQTVKMLRT QRPAMVQTED EYQFCYQAAL EYLGSFDHYA
1501T
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1501 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.