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PTPRS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTPRS Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTPRS antibodies →
$725.00each
Catalog Number: LS-G102216-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPRS (NM_130854), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Bptp-1 | Cryp-alpha | LAR-PTP2 | PTP NE-3 | PTP-varsigma | PTPSIGMA | R-PTP-S | PTP sigma | PTPRS | R-PTP-sigma
Origin Species
Human
NCBI GenBank
NM_002850
SwissProt
Q13332

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
209.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPTWGPGMV SVVGPMGLLV VLLVGGCAAE EPPRFIKEPK DQIGVSGGVA SFVCQATGDP
61KPRVTWNKKG KKVNSQRFET IEFDESAGAV LRIQPLRTPR DENVYECVAQ NSVGEITVHA
121KLTVLREDQL PSGFPNIDMG PQLKVVERTR TATMLCAASG NPDPEITWFK DFLPVDPSAS
181NGRIKQLRSG ALQIESSEET DQGKYECVAT NSAGVRYSSP ANLYVRVRRV APRFSILPMS
241HEIMPGGNVN ITCVAVGSPM PYVKWMQGAE DLTPEDDMPV GRNVLELTDV KDSANYTCVA
301MSSLGVIEAV AQITVKSLPK APGTPMVTEN TATSITITWD SGNPDPVSYY VIEYKSKSQD
361GPYQIKEDIT TTRYSIGGLS PNSEYEIWVS AVNSIGQGPP SESVVTRTGE QAPASAPRNV
421QARMLSATTM IVQWEEPVEP NGLIRGYRVY YTMEPEHPVG NWQKHNVDDS LLTTVGSLLE
481DETYTVRVLA FTSVGDGPLS DPIQVKTQQG VPGQPMNLRA EARSETSITL SWSPPRQESI
541IKYELLFREG DHGREVGRTF DPTTSYVVED LKPNTEYAFR LAARSPQGLG AFTPVVRQRT
601LQSKPSAPPQ DVKCVSVRST AILVSWRPPP PETHNGALVG YSVRYRPLGS EDPEPKEVNG
661IPPTTTQILL EALEKWTQYR ITTVAHTEVG PGPESSPVVV RTDEDVPSAP PRKVEAEALN
721ATAIRVLWRS PAPGRQHGQI RGYQVHYVRM EGAEARGPPR IKDVMLADAQ EMVITNLQPE
781TAYSITVAAY TMKGDGARSK PKVVVTKGAV LGRPTLSVQQ TPEGSLLARW EPPAGTAEDQ
841VLGYRLQFGR EDSTPLATLE FPPSEDRYTA SGVHKGATYV FRLAARSRGG LGEEAAEVLS
901IPEDTPRGHP QILEAAGNAS AGTVLLRWLP PVPAERNGAI VKYTVAVREA GALGPARETE
961LPAAAEPGAE NALTLQGLKP DTAYDLQVRA HTRRGPGPFS PPVRYRTFLR DQVSPKNFKV
1021KMIMKTSVLL SWEFPDNYNS PTPYKIQYNG LTLDVDGRTT KKLITHLKPH TFYNFVLTNR
1081GSSLGGLQQT VTAWTAFNLL NGKPSVAPKP DADGFIMVYL PDGQSPVPVQ SYFIVMVPLR
1141KSRGGQFLTP LGSPEDMDLE ELIQDISRLQ RRSLRHSRQL EVPRPYIAAR FSVLPPTFHP
1201GDQKQYGGFD NRGLEPGHRY VLFVLAVLQK SEPTFAASPF SDPFQLDNPD PQPIVDGEEG
1261LIWVIGPVLA VVFIICIVIA ILLYKNKPDS KRKDSEPRTK CLLNNADLAP HHPKDPVEMR
1321RINFQTPGML SHPPIPIADM AEHTERLKAN DSLKLSQEYE SIDPGQQFTW EHSNLEVNKP
1381KNRYANVIAY DHSRVILQPI EGIMGSDYIN ANYVDGYRRQ NAYIATQGPL PETFGDFWRM
1441VWEQRSATIV MMTRLEEKSR IKCDQYWPNR GTETYGFIQV TLLDTIELAT FCVRTFSLHK
1501NGSSEKREVR QFQFTAWPDH GVPEYPTPFL AFLRRVKTCN PPDAGPIVVH CSAGVGRTGC
1561FIVIDAMLER IKPEKTVDVY GHVTLMRSQR NYMVQTEDQY SFIHEALLEA VGCGNTEVPA
1621RSLYAYIQKL AQVEPGEHVT GMELEFKRLA NSKAHTSRFI SANLPCNKFK NRLVNIMPYE
1681STRVCLQPIR GVEGSDYINA SFIDGYRQQK AYIATQGPLA ETTEDFWRML WENNSTIVVM
1741LTKLREMGRE KCHQYWPAER SARYQYFVVD PMAEYNMPQY ILREFKVTDA RDGQSRTVRQ
1801FQFTDWPEQG VPKSGEGFID FIGQVHKTKE QFGQDGPISV HCSAGVGRTG VFITLSIVLE
1861RMRYEGVVDI FQTVKMLRTQ RPAMVQTEDE YQFCYQAALE YLGSFDHYAT
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1910 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.