Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POLL / DNA Polymerase Lambda Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129891-100
Catalog Number: LS-G129891
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNA Polymerase lambda(POLL) (NM_001174085), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DNA polymerase beta-2 | DNA polymerase beta-N | DNA polymerase lambda | DNA polymerase kappa | POLL | Pol beta2 | BETAN | Pol Lambda | POLKAPPA
- Origin Species
- Human
- NCBI GenBank
- NM_013274
- SwissProt
- Q9UGP5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPRGILKAF PKRQKIHADA SSKVLAKIPR REEGEEAEEW LSSLRAHVVR TGIGRARAEL61FEKQIVQHGG QLCPAQGPGV THIVVDEGMD YERALRLLRL PQLPPGAQLV KSAWLSLCLQ121ERRLVDVAGF SIFIPSRYLD HPQPSKAEQD ASIPPGTHEA LLQTALSPPP PPTRPVSPPQ181KAKEAPNTQA QPISDDEASD GEETQEACSI PGIGKRMAEK IIEILESGHL RKLDHISESV241PVLELFSNIW GAGTKTAQMW YQQGFRSLED IRSQASLTTQ QAIGLKHYSD FLERMPREEA301TEIEQTVQKA AQAFNSGLLC VACGSYRRGK ATCGDVDVLI THPDGRSHRG IFSRLLDSLR361QEGFLTDDLV SQEENGQQQK YLGVCRLPGP GRRHRRLDII VVPYSEFACA LLYFTGSAHF421NRSMRALAKT KGMSLSEHAL STAVVRNTHG CKVGPGRVLP TPTEKDVFRL LGLPYREPAE481RDWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
483 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.