POLL / DNA Polymerase Lambda Antibody
Catalog Number: LS-C409943
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human POLL / DNA Polymerase Lambda Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G85322-20
Catalog Number: LS-G85322
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DNA Polymerase lambda(POLL) (NM_001174085) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DNA polymerase beta-2 | DNA polymerase beta-N | DNA polymerase lambda | DNA polymerase kappa | POLL | Pol beta2 | BETAN | Pol Lambda | POLKAPPA
- Origin Species
- Human
- NCBI GenBank
- NM_013274
- SwissProt
- Q9UGP5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPRGILKAF PKRQKIHADA SSKVLAKIPR REEGEEAEEW LSSLRAHVVR TGIGRARAEL61FEKQIVQHGG QLCPAQGPGV THIVVDEGMD YERALRLLRL PQLPPGAQLV KSAWLSLCLQ121ERRLVDVAGF SIFIPSRYLD HPQPSKAEQD ASIPPGTHEA LLQTALSPPP PPTRPVSPPQ181KAKEAPNTQA QPISDDEASD GEETQEACSI PGIGKRMAEK IIEILESGHL RKLDHISESV241PVLELFSNIW GAGTKTAQMW YQQGFRSLED IRSQASLTTQ QAIGLKHYSD FLERMPREEA301TEIEQTVQKA AQAFNSGLLC VACGSYRRGK ATCGDVDVLI THPDGRSHRG IFSRLLDSLR361QEGFLTDDLV SQEENGQQQK YLGVCRLPGP GRRHRRLDII VVPYSEFACA LLYFTGSAHF421NRSMRALAKT KGMSLSEHAL STAVVRNTHG CKVGPGRVLP TPTEKDVFRL LGLPYREPAE481RDWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
483 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
