POLL / DNA Polymerase Lambda Antibody
Catalog Number: LS-C409943
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Human POLL / DNA Polymerase Lambda Protein (Recombinant His)
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| Expression System | Escherichia coli (Prokaryotic) |
| Format: | Liquid |
$282.00each
Catalog Number: LS-G1475-10
Catalog Number: LS-G1475
For bulk orders or custom formulations:
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- Description
- DNA polymerase lambda, also known as POLL, is a DNA polymerase involved in base excision repair (BER). POLL is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. In addition, POLL may play a role in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A. Recombinant human POLL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Category
- Proteins & Peptides > Proteins > Recombinant Proteins
- Alternative Names
- DNA polymerase beta-2 | DNA polymerase beta-N | DNA polymerase lambda | DNA polymerase kappa | POLL | Pol beta2 | BETAN | Pol Lambda | POLKAPPA
- Origin Species
- Human
- NCBI GenBank
- NM_013274|NP_037406.1
- SwissProt
- Q9UGP5
Protein
- Expression System
- Escherichia coli (Prokaryotic)
- Fusion Tag
- Hexahistidine Tag
- Protein Type
- Recombinant
- Amino Acid Sequence
11-300aa|MG SSHHHHHHSS GLVPRGSHML MHHQKYLQRF LGGKREKKQK EACSIPGIGK61RMAEKIIEIL ESGHLRKLDH ISESVPVLEL FSNIWGAGTK TAQMWYQQGF RSLEDIRSQA121SLTTQQAIGL KHYSDFLERM PREEATEIEQ TVQKAAQAFN SGLLCVACGS YRRGKATCGD181VDVLITHPDG RSHRGIFSRL LDSLRQEGFL TDDLVSQEEN GQQQKYLGVC RLPGPGRRHR241RLDIIVVPYS EFACALLYFT GSAHFNRSMR ALAKTKGMSL SEHALSTAVV RNTHGCKVGP301GRVLPTPTEK DVFRLLGLPY REPAERDW
328 aa
Format
- Purity
- >90%
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl.
- Purity Measurement Method
- SDS-PAGE (Coomassie Stain)
Storage & Handling
- Storage Conditions
- Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
