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PODN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PODN Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PODN antibodies →
$504.00each
Catalog Number: LS-G131066-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PODN (NM_001199080), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Podocan proteoglycan | Podocan | SLRR5A | PCAN | PODN
Origin Species
Human
NCBI GenBank
NM_153703
SwissProt
Q7Z5L7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGEEAEQPA WFRQPWRPGA SDSAPPAGTM AQSRVLLLLL LLPPQLHLGP VLAVRAPGFG
61RSGGHSLSPE ENEFAEEEPV LVLSPEEPGP GPAAVSCPRD CACSQEGVVD CGGIDLREFP
121GDLPEHTNHL SLQNNQLEKI YPEELSRLHR LETLNLQNNR LTSRGLPEKA FEHLTNLNYL
181YLANNKLTLA PRFLPNALIS VDFAANYLTK IYGLTFGQKP NLRSVYLHNN KLADAGLPDN
241MFNGSSNVEV LILSSNFLRH VPKHLPPALY KLHLKNNKLE KIPPGAFSEL SSLRELYLQN
301NYLTDEGLDN ETFWKLSSLE YLDLSSNNLS RVPAGLPRSL VLLHLEKNAI RSVDANVLTP
361IRSLEYLLLH SNQLREQGIH PLAFQGLKRL HTVHLYNNAL ERVPSGLPRR VRTLMILHNQ
421ITGIGREDFA TTYFLEELNL SYNRITSPQV HRDAFRKLRL LRSLDLSGNR LHTLPPGLPR
481NVHVLKVKRN ELAALARGAL VGMAQLRELY LTSNRLRSRA LGPRAWVDLA HLQLLDIAGN
541QLTEIPEGLP ESLEYLYLQN NKISAVPANA FDSTPNLKGI FLRFNKLAVG SVVDSAFRRL
601KHLQVLDIEG NLEFGDISKD RGRLGKEKEE EEEEEEEEEE TR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

642 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.