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PODN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PODN Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PODN antibodies →
$504.00each
Catalog Number: LS-G105700-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PODN (NM_153703), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Podocan proteoglycan | Podocan | SLRR5A | PCAN | PODN
Origin Species
Human
NCBI GenBank
NM_153703
SwissProt
Q7Z5L7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGARARGAQ LRLGERVRPV GRRSAPGRSR FHQPWRPGAS DSAPPAGTMA QSRVLLLLLL
61LPPQLHLGPV LAVRAPGFGR SGGHSLSPEE NEFAEEEPVL VLSPEEPGPG PAAVSCPRDC
121ACSQEGVVDC GGIDLREFPG DLPEHTNHLS LQNNQLEKIY PEELSRLHRL ETLNLQNNRL
181TSRGLPEKAF EHLTNLNYLY LANNKLTLAP RFLPNALISV DFAANYLTKI YGLTFGQKPN
241LRSVYLHNNK LADAGLPDNM FNGSSNVEVL ILSSNFLRHV PKHLPPALYK LHLKNNKLEK
301IPPGAFSELS SLRELYLQNN YLTDEGLDNE TFWKLSSLEY LDLSSNNLSR VPAGLPRSLV
361LLHLEKNAIR SVDANVLTPI RSLEYLLLHS NQLREQGIHP LAFQGLKRLH TVHLYNNALE
421RVPSGLPRRV RTLMILHNQI TGIGREDFAT TYFLEELNLS YNRITSPQVH RDAFRKLRLL
481RSLDLSGNRL HTLPPGLPRN VHVLKVKRNE LAALARGALA GMAQLRELYL TSNRLRSRAL
541GPRAWVDLAH LQLLDIAGNQ LTEIPEGLPE SLEYLYLQNN KISAVPANAF DSTPNLKGIF
601LRFNKLAVGS VVDSAFRRLK HLQVLDIEGN LEFGDISKDR GRLGKEKEEE EEEEEEEEET
661R
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

661 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.