PODN Antibody
Catalog Number: LS-C411852
2 sizes / from $328.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PODN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93106-20
Catalog Number: LS-G93106
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PODN (NM_001199082) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Podocan proteoglycan | Podocan | SLRR5A | PCAN | PODN
- Origin Species
- Human
- NCBI GenBank
- NM_153703
- SwissProt
- Q7Z5L7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGARARGAQ LRLGERVRPV GRRSAPGRSR FRQPWRPGAS DSAPPAGTMA QSRVLLLLLL61LPPQLHLGPV LAVRAPGFGR SGGHSLSPEE NEFAEEEPVL VLSPEEPGPG PAAVSCPRDC121ACSQEGVVDC GGIDLREFPG DLPEHTNHLS LQNNKLEKIP PGAFSELSSL RELYLQNNYL181TDEGLDNETF WKLSSLEYLD LSSNNLSRVP AGLPRSLVLL HLEKNAIRSV DANVLTPIRS241LEYLLLHSNQ LREQGIHPLA FQGLKRLHTV HLYNNALERV PSGLPRRVRT LMILHNQITG301IGREDFATTY FLEELNLSYN RITSPQVHRD AFRKLRLLRS LDLSGNRLHT LPPGLPRNVH361VLKVKRNELA ALARGALVGM AQLRELYLTS NRLRSRALGP RAWVDLAHLQ LLDIAGNQLT421EIPEGLPESL EYLYLQNNKI SAVPANAFDS TPNLKGIFLR FNKLAVGSVV DSAFRRLKHL481QVLDIEGNLE FGDISKDRGR LGKEKEEEEE EEEEEEETRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
519 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
