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OPA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OPA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OPA1 antibodies →
$725.00each
Catalog Number: LS-G107618-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OPA1 (NM_130836), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0567 | MGM1 | LargeG | OPA1 | NPG | NTG | Optic atrophy protein 1
Origin Species
Human
NCBI GenBank
AB011139
SwissProt
O60313

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWRLRRAAVA CEVCQSLVKH SSGIKGSLPL QKLHLVSRSI YHSHHPTLKL QRPQLRTSFQ
61QFSSLTNLPL RKLKFSPIKY GYQPRRNFWP ARLATRLLKL RYLILGSAVG GGYTAKKTFD
121QWKDMIPDLS EYKWIVPDIV WEIDEYIDFE KIRKALPSSE DLVKLAPDFD KIVESLSLLK
181DFFTSGSPEE TAFRATDRGS ESDKHFRKGL LGELILLQQQ IQEHEEEARR AAGQYSTSYA
241QQKRKVSDKE KIDQLQEELL HTQLKYQRIL ERLEKENKEL RKLVLQKDDK GIHHRKLKKS
301LIDMYSEVLD VLSDYDASYN TQDHLPRVVV VGDQSAGKTS VLEMIAQARI FPRGSGEMMT
361RSPVKVTLSE GPHHVALFKD SSREFDLTKE EDLAALRHEI ELRMRKNVKE GCTVSPETIS
421LNVKGPGLQR MVLVDLPGVI NTVTSGMAPD TKETIFSISK AYMQNPNAII LCIQDGSVDA
481ERSIVTDLVS QMDPHGRRTI FVLTKVDLAE KNVASPSRIQ QIIEGKLFPM KALGYFAVVT
541GKGNSSESIE AIREYEEEFF QNSKLLKTSM LKAHQVTTRN LSLAVSDCFW KMVRESVEQQ
601ADSFKATRFN LETEWKNNYP RLRELDRNEL FEKAKNEILD EVISLSQVTP KHWEEILQQS
661LWERVSTHVI ENIYLPAAQT MNSGTFNTTV DIKLKQWTDK QLPNKAVEVA WETLQEEFSR
721FMTEPKGKEH DDIFDKLKEA VKEESIKRHK WNDFAEDSLR VIQHNALEDR SISDKQQWDA
781AIYFMEEALQ ARLKDTENAI ENMVGPDWKK RWLYWKNRTQ EQCVHNETKN ELEKMLKCNE
841EHPAYLASDE ITTVRKNLES RGVEVDPSLI KDTWHQVYRR HFLKTALNHC NLCRRGFYYY
901QRHFVDSELE CNDVVLFWRI QRMLAITANT LRQQLTNTEV RRLEKNVKEV LEDFAEDGEK
961KIKLLTGKRV QLAEDLKKVR EIQEKLDAFI EALHQEKSGP
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1000 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.