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OPA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OPA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OPA1 antibodies →
$725.00each
Catalog Number: LS-G107616-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OPA1 (NM_130834), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0567 | MGM1 | LargeG | OPA1 | NPG | NTG | Optic atrophy protein 1
Origin Species
Human
NCBI GenBank
AB011139
SwissProt
O60313

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWRLRRAAVA CEVCQSLVKH SSGIKGSLPL QKLHLVSRSI YHSHHPTLKL QRPQLRTSFQ
61QFSSLTNLPL RKLKFSPIKY GYQPRRNFWP ARLATRLLKL RYLILGSAVG GGYTAKKTFD
121QWKDMIPDLS EYKWIVPDIV WEIDEYIDFE KIRKALPSSE DLVKLAPDFD KIVESLSLLK
181DFFTSGHKLV SEVIGASDLL LLLGSPEETA FRATDRGSES DKHFRKVSDK EKIDQLQEEL
241LHTQLKYQRI LERLEKENKE LRKLVLQKDD KGIHHRKLKK SLIDMYSEVL DVLSDYDASY
301NTQDHLPRVV VVGDQSAGKT SVLEMIAQAR IFPRGSGEMM TRSPVKVTLS EGPHHVALFK
361DSSREFDLTK EEDLAALRHE IELRMRKNVK EGCTVSPETI SLNVKGPGLQ RMVLVDLPGV
421INTVTSGMAP DTKETIFSIS KAYMQNPNAI ILCIQDGSVD AERSIVTDLV SQMDPHGRRT
481IFVLTKVDLA EKNVASPSRI QQIIEGKLFP MKALGYFAVV TGKGNSSESI EAIREYEEEF
541FQNSKLLKTS MLKAHQVTTR NLSLAVSDCF WKMVRESVEQ QADSFKATRF NLETEWKNNY
601PRLRELDRNE LFEKAKNEIL DEVISLSQVT PKHWEEILQQ SLWERVSTHV IENIYLPAAQ
661TMNSGTFNTT VDIKLKQWTD KQLPNKAVEV AWETLQEEFS RFMTEPKGKE HDDIFDKLKE
721AVKEESIKRH KWNDFAEDSL RVIQHNALED RSISDKQQWD AAIYFMEEAL QARLKDTENA
781IENMVGPDWK KRWLYWKNRT QEQCVHNETK NELEKMLKCN EEHPAYLASD EITTVRKNLE
841SRGVEVDPSL IKDTWHQVYR RHFLKTALNH CNLCRRGFYY YQRHFVDSEL ECNDVVLFWR
901IQRMLAITAN TLRQQLTNTE VRRLEKNVKE VLEDFAEDGE KKIKLLTGKR VQLAEDLKKV
961REIQEKLDAF IEALHQEKSG P
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

981 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.