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OPA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OPA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OPA1 antibodies →
$725.00each
Catalog Number: LS-G107613-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OPA1 (NM_130831), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0567 | MGM1 | LargeG | OPA1 | NPG | NTG | Optic atrophy protein 1
Origin Species
Human
NCBI GenBank
AB011139
SwissProt
O60313

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
107.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWRLRRAAVA CEVCQSLVKH SSGIKGSLPL QKLHLVSRSI YHSHHPTLKL QRPQLRTSFQ
61QFSSLTNLPL RKLKFSPIKY GYQPRRNFWP ARLATRLLKL RYLILGSAVG GGYTAKKTFD
121QWKDMIPDLS EYKWIVPDIV WEIDEYIDFG SPEETAFRAT DRGSESDKHF RKVSDKEKID
181QLQEELLHTQ LKYQRILERL EKENKELRKL VLQKDDKGIH HRKLKKSLID MYSEVLDVLS
241DYDASYNTQD HLPRVVVVGD QSAGKTSVLE MIAQARIFPR GSGEMMTRSP VKVTLSEGPH
301HVALFKDSSR EFDLTKEEDL AALRHEIELR MRKNVKEGCT VSPETISLNV KGPGLQRMVL
361VDLPGVINTV TSGMAPDTKE TIFSISKAYM QNPNAIILCI QDGSVDAERS IVTDLVSQMD
421PHGRRTIFVL TKVDLAEKNV ASPSRIQQII EGKLFPMKAL GYFAVVTGKG NSSESIEAIR
481EYEEEFFQNS KLLKTSMLKA HQVTTRNLSL AVSDCFWKMV RESVEQQADS FKATRFNLET
541EWKNNYPRLR ELDRNELFEK AKNEILDEVI SLSQVTPKHW EEILQQSLWE RVSTHVIENI
601YLPAAQTMNS GTFNTTVDIK LKQWTDKQLP NKAVEVAWET LQEEFSRFMT EPKGKEHDDI
661FDKLKEAVKE ESIKRHKWND FAEDSLRVIQ HNALEDRSIS DKQQWDAAIY FMEEALQARL
721KDTENAIENM VGPDWKKRWL YWKNRTQEQC VHNETKNELE KMLKCNEEHP AYLASDEITT
781VRKNLESRGV EVDPSLIKDT WHQVYRRHFL KTALNHCNLC RRGFYYYQRH FVDSELECND
841VVLFWRIQRM LAITANTLRQ QLTNTEVRRL EKNVKEVLED FAEDGEKKIK LLTGKRVQLA
901EDLKKVREIQ EKLDAFIEAL HQEK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

924 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.