Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NPHP1 / Nephronophthisis Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124765-100
Catalog Number: LS-G124765
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Nephronophthisis(NPHP1) (NM_001128179), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- JBTS4 | Nephrocystin 1 | Nephronophthisis | SLSN1 | Nephrocystin-1 | Nephronophthisis 1 (juvenile) | NPH1 | NPHP1
- Origin Species
- Human
- NCBI GenBank
- NM_000272
- SwissProt
- O15259
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLARRQRDPL QALRRRNQEL KQQVDSLLSE SQLKEALEPN KRQHIYQRVG APTEEEEESE61SEDSEDSGGE EEDAEEEEEE KEENESHKWS TGEEYIAVGD FTAQQVGDLT FKKGEILLVI121EKKPDGWWIA KDAKGNEGLV PRTYLEPYSE EEEGQESSEE GSEEDVEAVD ETADGAEVKQ181RTDPHWSAVQ KAISEINTVD VLTTMGAIPA GFRPSTLSQL LEEGNQFRAN YFLQPELMPS241QLAFRDLMWD ATEGTIRSRP SRISLILTLW SCKMIPLPGM SIQVLSRHVR LCLFDGNKVL301SNIHTVRATW QPKKPKTWTF SPQVTRILPC LLDGDCFIRS NSASPDLGIL FELGISYIRN361STGERGELSC GWVFLKLFDA SGVPIPAKTY ELFLNGGTPY EKGIEVDPSI SRRAHGSVFY421QIMTMRRQPQ LLVKLRSLNR RSRNVLSLLP ETLIGNMCSI HLLIFYRQIL GDVLLKDRMS481LQSTDLISHP MLATFPMLLE QPDVMDALRS SWAGKESTLK RSEKRDKEFL KSTFLLVYHD541CVLPLLHSTR LPPFRWAEEE TETARWKVIT DFLKQNQENQ GALQALLSPD GVHEPFDLSE601QTYDFLGEMR KNAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
614 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.